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BJ5183

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Alternate Names

BJ5183 is a component of Strategene's AdEasy system for construction of Adenovirus vectors.

Sources

Chromosomal Genotype

This genotype is inferred from the description in Takahashi et al. [1] as:

recB recC sbcB sbcC endA galK met thi-1 bioT hsdR rpsL(strR)

The nature of bioT is unclear, as this is not a listed synonym for any annotated E. coli gene.

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Notes

BJ5183 is a recBC sbc strain used for doing homologous recombination with linear dsDNA fragments. It has been used for construction of gene therapy vectors[2] and combining HSV-1 cosmids[3]. The strain originates with D. Hanahan, but it's construction is not well documented. The recombination properties of BJ5183 have been examined by Takahashi et al. [1].

References

See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Takahashi, N et al. (2003) An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene 303 89-97 PubMed EcoliWiki page
  2. Renaut, L et al. (2002) A rapid and easy method for production and selection of recombinant adenovirus genomes. J. Virol. Methods 100 121-31 PubMed EcoliWiki page
  3. Kong, Y et al. (1999) An efficient in vivo recombination cloning procedure for modifying and combining HSV-1 cosmids. J. Virol. Methods 80 129-36 PubMed EcoliWiki page