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BL21(DE3)pLysS

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Alternate Names

Sources

Promega Stratagene

Chromosomal Genotype

ompT, hsdSB(rB-rB-), dcm, gal

Plasmids

pLysS

Phage

λDE3[1]

Phenotypes

Cmr

Construction

Notes

Contains an IPTG-inducible gene for T7 RNA polymerase[2]

References

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  1. Studier, FW & Moffatt, BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189 113-30 PubMed EcoliWiki page
  2. Davanloo, P et al. (1984) Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 81 2035-9 PubMed EcoliWiki page