BW25113 - EcoliWiki
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BW25113

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Chromosomal Genotype HelpIcon.jpg

(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514

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Notes

BW25113 was used to make the Keio Collection of single-gene knockouts. [1] [2] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [2] said the strain is lacIQ, but it was later shown to be lacI+ (Supplementary Table 1)[1].

References

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  1. 1.0 1.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  2. 2.0 2.1 Datsenko KA & Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97: 6640-5 PubMed EcoliWiki page
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