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Category:Genes in the ASKA clone collection
From EcoliWiki
The ASKA clone set is an ORFeome collection for E. coli K-12[1]. ORFs were amplified by PCR and cloned between SfiI restriction sites. The ASKA plasmids use a high copy number plasmid vector, and have
- IPTG-inducible expression of the cloned ORF controlled by a copy of lacIq on the plasmid
- N-terminal Histidine tag
- in-frame C-terminal fusion to GFP
The ASKA set has been used for a wide variety of genomics applications, including complementation, protein interaction screens[2], localization studies, and overexpression phenotypes.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
- ↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
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