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Category:Genes not mapped to a genome sequence

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This page has a list of genes that are described in the 1998 linkage map for E. coli K-12 (Edition 10)[1] but have not been correlated with a gene in EcoliWiki. In most cases, the alleles that led to assignment of these gene names are likely to affect known genes, but which gene has not been determined.


Further information about a particular gene may be found on the EcoliWiki page for the publication(s) describing the mutants. Click on the PMID: link to access that page.

Gene Approx. Map Location (Min) Mnemonic Paper(s) Describing Gene Notes Candidate Gene(s)

abpS

63.5

Arg binding protein

Low-affinity transport system for arginine and ornithine; periplasmic binding protein CGSC

abs

94.1

Antibiotic sensitivity

Sensitivity and permeability to antibiotics and dyes CGSC

acpX

43.9

Acyl carrier protein

acpS; originally thought to be holo-ACP synthase; perhaps cryptic second gene or regulator CGSC

acrC

4.5

Acridine

Sensitivity to acriflavine; transmembrane protein CGSC

adhB

19.1

Alcohol dehydrogenase

Alcohol dehydrogenase CGSC

aga

70.7

Acetylgalactosamine

Cluster of putative N-acetylgalactosamine pathway genes, including the kba gene, and mannose permease homologs CGSC

aroI

84.2

Aromatic

Function unknown CGSC

azaA

44.6

Azaserine

Mutants azaserine resistant CGSC

azaB

71.9

Azaserine

Mutants azaserine resistant CGSC

azl

58.1

Azaleucine

Mutants azaleucine resistant; regulates ilv and leu CGSC

bfm

85.9

BF23 multiplication

Controls phage BF23 multiplication CGSC

bglT

84.9

-Glucoside

bglE; regulatory gene for BglA CGSC

bioP

86.6

Biotin

bir, birB; biotin transport CGSC

brnR

8.5

Branched chain

PMID:4590465[2]

Mutants valine resistant, glycylvaline sensitive CGSC

brnS

1.2

Branched chain

PMID:4590465[2]

Mutants valine resistant, glycylvaline sensitive CGSC

brnT

65.2

Branched chain

Low-affinity transport of Ile CGSC

bymA

93.2

Bypass maltose

Growth on maltose in MalT cells CGSC

calA

95.0

Calcium

Calcium-proton antiport activity CGSC

calC

15.2

Calcium

Calcium transport; mutants defective in chemotaxis CGSC

calD

9.4

Calcium

Calcium transport; mutants defective in chemotaxis CGSC

cdsS

71.7

CDP diglyceride synthase

Stability of CDP diglyceride synthase CGSC

cpsA

45.4

Capsular polysaccharide synthesis

Colanic acid (CPS) biosynthesis CGSC

cpsC

45.8

Capsular polysaccharide synthesis

Colanic acid (CPS) biosynthesis CGSC

cpsD

45.8

Capsular polysaccharide synthesis

Colanic acid (CPS) biosynthesis CGSC

cpsE

45.8

Capsular polysaccharide synthesis

Colanic acid (CPS) biosynthesis CGSC

cpsF

90.2

Capsular polysaccharide synthesis

Colanic acid (CPS) biosynthesis CGSC

cpxB

41.3

Conjugative plasmid expression

Phage Q resistance, membrane protein CGSC

crg

28.8

Cold resistant growth

Allows cold-resistant growth CGSC

csiA

85.3

Carbon starvation induced

Stationary phase inducible protein CGSC

csiB

17.4

Carbon starvation induced

Stationary phase inducible protein CGSC

csiC

66.0

Carbon starvation induced

Stationary phase inducible protein CGSC

csiF

8.6

Carbon starvation induced

Stationary phase inducible protein CGSC

cup

97.0

Carbohydrate uptake

Mutants have defective carbohydrate uptake CGSC

cxm

6.3

Carbon-xylose metabolism

cxr; methyl glyoxal synthesis; D-xylose utilization CGSC

cysX

81.5

Cysteine

Reading frame in opposite orientation within cysE gene; polypeptide synthesized in maxicells CGSC

dadQ

98.9

D-Amino acid dehydrogenase

alnR; regulator of dad regulon CGSC

dctB

16.4

Dicarboxylic acid transport

Uptake of C-4 dicarboxylic acids; 3-fluoromalate resistance, D-tartrate resistant CGSC

del

64.3

Deletion

Affects frequency of IS1-mediated deletions; 1,000-fold reduction in deletion frequency CGSC

dgkR

93.7

Diglyceride kinase

Regulatory CGSC

dinY

41.9

Damage inducible

Repair gene CGSC

dnaI

40.3

DNA

DNA biosynthesis CGSC

dppG

14.0

Dipeptide permease

Uptake of dipeptides; dipeptide permease CGSC

dvl

7.4

Dye-visible light?

Sensitivity to SDS and toluidine blue plus light CGSC

ebgB

69.5

Evolved -galactosidase

Cryptic -galactoside utilization, possible paralog of lacY CGSC

envN

4.2

Envelope

Affects envelope; defects osmotically remedied CGSC

envP

90.4

Envelope

Affects envelope; defects osmotically remedied CGSC

envQ

60.7

Envelope

Affects envelope; defects osmotically remedied CGSC

envT

14.2

Envelope

Affects envelope; defects osmotically remedied CGSC

epp

E-pentapeptide

Minigene within 23S rRNA encoding functional pentapeptide; erythromycin resistance CGSC

esp

17.3

Efficiency site for phage

Site for efficient packaging of phage T1 CGSC

exbC

61.5

Export

Uptake of enterochelin; resistance or sensitivity to colicins CGSC

expA

22.2

Export

Expression of a group of export proteins CGSC

fatA

71.7

Fatty acid

Utilization of trans unsaturated fatty acids CGSC

fcsA

86.9

Filamentous, cold sensitive

Cold-sensitive cell division mutant CGSC

fexB

86.7

F exclusion

Affects ArcA phenotype CGSC

fhlB

95.1

Formate hydrogen-lyase

Formate hydrogen-lyase; activated by fhlA CGSC

fipB

86.0

F1 phage

Morphogenesis of phage F1 CGSC

fipC

75.0

F1 phage

Morphogenesis of phage F1 CGSC

flkB

95.4

FKBP-like

Periplasmic PPIase, of FK506-binding protein type (EC 5.2.1.8); contains pipecolic acid residue CGSC

gadR

79.6

Glutamate deCase

Regulatory gene for gat? CGSC

garA

15.8

Glucarate

D-Glucarate utilization CGSC

garB

3.6

Glucarate

D-Glucarate utilization CGSC

glmX

26.2

Glucosamine

Affects suppression of glmS mutations by nagB CGSC

gltE

81.4

Glutamate

Glutamyl-tRNA synthetase; possible regulatory subunit CGSC

gltH

21.8

Glutamate

Growth on glutamate CGSC

gltR

92.3

Glutamate

Growth on glutamate at 42C CGSC

gprA

0.3

Growth of phage, replication

Replication of certain lambdoid phage CGSC

gprB

0.2

Growth of phage, replication

Replication of certain lambdoid phage CGSC

gurC

18.0

Glucuronide

Utilization of glucuronides CGSC

het

84.6

Heterogeneous size

cop; possibly structural gene for DNA-binding protein; near ori CGSC

hslC

19.9

Heat shock locus

Protein expressed as heat shock regulon member CGSC

hslD

24.1

Heat shock locus

Protein expressed as heat shock regulon member CGSC

hslK

40.7

Heat shock locus

Protein expressed as heat shock regulon member CGSC

hslW

94.2

Heat shock locus

Heat-inducible; regulatory gene, near groE CGSC

ilvF

57.0

Isoleucine-valine (requirement)

Production of valine-resistant acetolactate synthase activity CGSC

ilvR

99.9

Isoleucine-valine

CGSC

ilvU

6.4

Isoleucine-valine (requirement)

Regulation of ileS and modification of Ile tRNA2 and Val tRNA2 CGSC

inm

79.0

Insensitive NG mutagenesis

Susceptibility to mutagenesis by nitrosoguanidine CGSC

isfA

86.0

Inhibits SOS function

Regulatory gene; SOS-related CGSC

ksgB

37.7

Kasugamycin

High-level resistance to kasugamycin CGSC

ksgC

12.0

Kasugamycin

Resistance to kasugamycin; affects ribosomal protein S2 CGSC

ksgD

30.9

Kasugamycin

Resistance to kasugamycin CGSC

leuJ

13.7

Leucine (biosynthesis)

flr; regulation of leu and ilv operons CGSC

leuR

79.3

Leucine

Regulates level of leucyl-tRNA synthetase CGSC

leuY

9.5

Leucine

Regulates level of leucyl-tRNA synthetase CGSC

lev

9.0

Levallorphan

Resistance to levallorphan CGSC

linB

29.3

Lincomycin

High-level lincomycin resistance CGSC

lpcB

68.0

Lipopolysaccharide core

mrc, pon; T-phage resistance, novobiocin sensitivity CGSC

lrb

7.3

L-Ribose (utilization)

Affects NADPH-linked L-ribose reductase activity CGSC

lysX

63.3

Lysine

Lysine excretion CGSC

lytA

60.6

Lytic

Tolerance to -lactams; autolysis CGSC

mafA

0.9

Maintenance of F

PMID:327277PMID:391803[3]

Maintenance of F-like plasmids CGSC

mafB

1.9

Maintenance of F

PMID:391803[3]

Maintenance of F-like plasmids CGSC

mbrB

88.6

Mothball resistant

PMID:2176633[4]

Resistance to camphor vapors; coupling of cell division and replication, growth rate and partitioning CGSC

meb

78.7

malE bypass

Suppressor of malE secB-defective transport of mal-binding protein CGSC

mglR

16.9

Methyl-galactoside

R-MG; regulatory gene CGSC

mng

40.0

Manganese

Manganese resistance CGSC

mraA

2.0

Murein cluster a

D-Alanine carboxypeptidase CGSC

mul

83.1

Mutability lambda

Mutability in UV-radiated lambda phage CGSC

murH

99.3

Murein

Terminal stage in peptidoglycan synthesis, incorporating disaccharide peptide units into wall CGSC

mutG

43.7

Mutator

Mutation causes high C-to-T mutation in second C of CCAGG; near but distinct from vsr; provisionally termed mutG CGSC

nalB

60.2

Nalidixic acid

Sensitivity to nalidixic acid (NAL) CGSC

neaB

75.0

Neamine

Neamine sensitivity CGSC

nfnA

80.8

Nitrofurantoin

Nitrofurantoin sensitivity CGSC

nfrD

54.2

N4 (phage) resistant

Phage N4 susceptibility CGSC

non

45.7

Nonmucoid

Affects capsule formation CGSC

oppE

98.9

Oligopeptide permease

Oligopeptide transport CGSC

opr

19.0

rpo reversed

Rate of degradation of aberrant RNAP-subunit proteins CGSC

ops

66.1

Overproduction of polysaccharide

Level of exopolysaccharide production CGSC

oriJ

30.5

Origin of replication

Locus in defective prophage rac CGSC

pac

31.0

Phenylacetate

Phenylacetate degradation CGSC

phxB

17.0

Phage X

Adsorption of X154 CGSC

poaR

65.9

Proline oxidase

Regulation of proline oxidase production CGSC

popD

0.2

Porphyrin

5-Aminolevulinate dehydratase (EC 4.2.1.24) CGSC

prlZ

71.4

Protein localization

Suppresses export defects in signal sequence mutations CGSC

proT

83.8

Proline

Proline transport carrier protein, putative CGSC

pus

20.6

Reverse of sup (amber)

Reverses accentuation effects of amber suppressor on relB mutations CGSC

qin

35.1

Q independent

kim; cryptic lambdoid phage CGSC

qmeC

75.3

Q membrane

Glycine resistance; penicillin sensitivity; membrane defect CGSC

qmeD

64.4

Q membrane

Glycine resistance; penicillin sensitivity; membrane defect CGSC

qmeE

37.9

Q membrane

Glycine resistance; penicillin sensitivity; membrane defect CGSC

ras

9.9

Radiation sensitive

Sensitive to X rays and UV CGSC

rdgA

16.1

RecA-dependent growth

Dependence of growth upon recA gene product CGSC

relX

62.8

Relaxed

Control of ppGpp synthesis CGSC

rer

89.9

Resistance to radiation

Resistance to UV and gamma radiation CGSC

ridA

73.1

Rifampin dependence

Rifampin resistance and dependence CGSC

ridB

85.5

Rifampin dependence

Transcription and translation; rifampin (rifamycin) sensitivity CGSC

rho

rimB

38.9

Ribosomal modification

50S ribosomal subunit maturation CGSC

rimC

26.2

Ribosomal modification

50S ribosomal subunit maturation CGSC

rimD

87.7

Ribosomal modification

50S ribosomal subunit maturation CGSC

rimE

74.1

Ribosomal modification

Ribosomal protein modification CGSC

rimH

13.6

Ribosomal modification

stsB; ribosomal modification CGSC

rit

89.2

Ribosomal thermolability

Affects thermolability of 50S ribosomal subunit CGSC

rorB

85.1

Roentgen resistance

Sensitivity to ionizing radiation, mitomycin C CGSC

sds

30.4

Suppresses disulfide bond

Motility defects CGSC

semA

40.5

Sensitivity to microcin

Sensitivity to microcin E492 CGSC

serR

2.3

Serine

Regulates level of seryl-tRNA synthetase CGSC

sfiC

25.8

Septum formation inhibition

PMID:3011740[5]

Element of e14, inhibitor of cell division CGSC

sipC

82.6

Suppressor of increased permeability

Mutations reverse the susceptibility to vancomycin and other hydrophobic antibiotics caused by TraT plasmid gene CGSC

sipD

82.7

Suppressor of increased permeability

Same phenotype as that produced by sipC CGSC

sir

61.2

SOS-independent repair

SOS-independent repair of mitomycin C-induced DNA damage CGSC

sloB

75.0

Slow growth

Tolerance for amidinopenicillin and nalidixic acid; slow growth rate CGSC

slrR

60.9

Sorbitol

gutR; regulatory gene for srl CGSC

srnA

9.4

Stable RNA

Degradation of stable RNA CGSC

ssaE

52.5

Suppression secA

Suppresses secA mutations CGSC

ssaG

41.8

Suppression secA

Suppresses secA mutations CGSC

ssaH

94.1

Suppression secA

Suppresses secA mutations CGSC

ssyD

3.0

Suppression of secY

Suppressor of secY mutation CGSC

stfZ

2.3

fts in reverse

PMID:8226652[6]

Antisense RNA blocks ftsZ mRNA translation, only inhibits cell division when overexpressed at high temperatures CGSC

stkA

77.5

Suppressor of transposase killing

Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC

stkB

86.1

Suppressor of transposase killing

Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC

stkC

98.5

Suppressor of transposase killing

Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC

stkD

28.6

Suppressor of transposase killing

Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC

strC

7.0

Streptomycin

strB; low-level streptomycin resistance; modifies ribosome structure CGSC

strM

78.3

Streptomycin

asuF?; control of ribosomal ambiguity CGSC

stsA

84.7

Altered RNase activity CGSC

suhA

78.4

Suppressor heat-shock proteins

Induction of heat shock genes CGSC

tabC

86.3

T4 abortion

Mutants fail to support growth of T4 CGSC

tanA

41.6

Trehalose, anaerobic

Anaerobic growth on trehalose CGSC

tanB

68.1

Trehalose, anaerobic

Anaerobic growth on trehalose CGSC

tdi

4.3

Transduction inhibition

Affects transduction, transformation, and rates of mutation CGSC

thdA

10.5

Thiophene degradation

Degradation of furans and thiophenes; may be tlnA? CGSC

psu

1.3 min

polarity suppressor

PMID:8380152[7]

hepA

thdC

94.2

Thiophene degradation

Degradation of furans and thiophenes CGSC

thdD

99.8

Thiophene degradation

Degradation of furans and thiophenes CGSC

ackB

39 min

acetate kinase

PMID:7033467[8]

Ack- mutants were defective in aerobic growth on acetate or glucose as well as acetate accumulation from glucose & mapped near 39' (ackA mapped to ~50'). CGSC

tlnA

10.5

Thiolutin

tlnI; resistance or sensitivity to thiolutin; may be thdA? CGSC

alaB

alanine-glutamate transaminase

PMID:2890623[9]

alaB was found to be capable of multi-copy suppression of an alanine auxotrophy.

tnm

92.0

Tn migration

Transposition of Tn9 and other transposons, development of phage Mu CGSC

dgd

D-galactose dehydrogenase

tolD

22.9

Tolerance

Bacteriocin tolerant; tolerance to colicins E2, E3, and ampicillin CGSC

treE

catabolic trehalose-6-phosphate phosphatase

PMID:8391102[10]

In the absence of osmotic stress, trehalose induces converstion to free glucose and a glucose polymer, in a pathway involving TreE.

tolE

22.9

Tolerance

Bacteriocin tolerant; tolerance to colicins E2, E3, and ampicillin CGSC

tolI

0.1

Tolerance

Bacteriocin tolerant; sensitivity to colicins Ia and Ib CGSC

tolJ

0.1

Tolerance

Bacteriocin tolerant; sensitivity to L, A, S4, E, and K CGSC

tsmA

40.1

Thymine suppression modifier

Affects suppression efficiency for nonsense and frameshift mutations of Thy strains CGSC

ups

27.1

Up-suppression

PMID:345090[11]

Increases efficiency of nonsense suppressorsCGSC

Proposed to be an allele of prfA[12]

uvh

90.2

UV hyperresistant to UVC

PMID:8988611[13]

Resistance to UVC, peroxide and antibiotics; may affect SOS repair CGSC

uvs

91.1

UV sensitivity

PMID:8988611[13]

UV-sensitive mutants, locus linked to uvr CGSC

mngK

between 34-37 min

PMID:4552988[14]

Manganese-resistance


References

See Help:References for how to manage references in EcoliWiki.

  1. Berlyn, MK (1998) Linkage map of Escherichia coli K-12, edition 10: the traditional map. Microbiol. Mol. Biol. Rev. 62 814-984 PubMed EcoliWiki page
  2. 2.0 2.1 Guardiola, J et al. (1974) Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J. Bacteriol. 117 393-405 PubMed EcoliWiki page
  3. 3.0 3.1 Wada, C & Yura, T (1979) Escherichia coli mutants incapable of supporting replication of F-like plasmids at high temperature: isolation and characterization of mafA and mafB mutants. J. Bacteriol. 140 864-73 PubMed EcoliWiki page
  4. Trun, NJ & Gottesman, S (1990) On the bacterial cell cycle: Escherichia coli mutants with altered ploidy. Genes Dev. 4 2036-47 PubMed EcoliWiki page
  5. Maguin, E et al. (1986) Reversibility of SOS-associated division inhibition in Escherichia coli. J. Bacteriol. 166 733-8 PubMed EcoliWiki page
  6. Dewar, SJ & Donachie, WD (1993) Antisense transcription of the ftsZ-ftsA gene junction inhibits cell division in Escherichia coli. J. Bacteriol. 175 7097-101 PubMed EcoliWiki page
  7. Ephrati-Elizur, E (1993) A mutation in a new gene of Escherichia coli, psu, requires secondary mutations for survival: psu mutants express a pleiotropic suppressor phenotype. J. Bacteriol. 175 207-13 PubMed EcoliWiki page
  8. Pascal, MC et al. (1981) Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism. J. Gen. Microbiol. 124 35-42 PubMed EcoliWiki page
  9. Wang, MD et al. (1987) Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids. J. Bacteriol. 169 5610-4 PubMed EcoliWiki page
  10. Strøm, AR & Kaasen, I (1993) Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol. Microbiol. 8 205-10 PubMed EcoliWiki page
  11. Davidoff-Abelson, R & Mindich, L (1978) A mutation that increases the activity of nonsense suppressors in Escherichia coli. Mol. Gen. Genet. 159 161-9 PubMed EcoliWiki page
  12. Riley, M (1993) Functions of the gene products of Escherichia coli. Microbiol. Rev. 57 862-952 PubMed EcoliWiki page
  13. 13.0 13.1 Ahmad, SI (1996) A mutant of Escherichia coli hyper-resistant to a number of DNA damaging agents: location of the mutational site. J. Photochem. Photobiol. B, Biol. 36 47-53 PubMed EcoliWiki page
  14. Silver, S et al. (1972) Manganese-resistant mutants of Escherichia coli: physiological and genetic studies. J. Bacteriol. 110 186-95 PubMed EcoliWiki page

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