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F-, DE(araD-araB)567, lacZ4787(del)::rrnB-3, LAM-, rph-1, DE(rhaD-rhaB)568, hsdR514
see description below
BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 (CGSC6159), which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 , a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113.
BW25113 was used to make the Keio Collection of single-gene knockouts.   Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al.  said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1).
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
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- Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed EcoliWiki page
- Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page
Pages in category "Strain:BW25113"
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