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Category:Strain:K-12 P2

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K-12 P2


pheA(del)::FRT tyrR(del)::PLtetO1-tyrAfbraroGfbr lacZ::PLtetO1-tyrAfbraroGfbr


tyrosine overproduction


PMID:17221195[1] describes construction of the deletion mutations

PMID:22869698[2] describes introduction of the fbr alleles into the chromosome




The tyrAfbr and aroGfbr alleles encode enzymes that are resistant to feedback inhibition.[1] The AroGfbrenzyme has an Asp-146-Asn substitution.[3]. The TyrAfbr enzyme has two amino acid substitutions: Met-53-Ile in the chorismate mutase domain and Ala-354-Val in the prephenate dehydrogenase domain.[4]

This strain is likely a derivative of K-12 MG1655, but it isn't explicitly stated in any of the papers.(Siegele (talk) 15:02, 11 July 2013 (CDT))


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  1. 1.0 1.1 Lütke-Eversloh, T & Stephanopoulos, G (2007) L-tyrosine production by deregulated strains of Escherichia coli. Appl. Microbiol. Biotechnol. 75 103-10 PubMed EcoliWiki page
  2. 2.0 2.1 Santos, CN et al. (2012) Rational, combinatorial, and genomic approaches for engineering L-tyrosine production in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 109 13538-43 PubMed EcoliWiki page
  3. Kikuchi, Y et al. (1997) Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. Appl. Environ. Microbiol. 63 761-2 PubMed EcoliWiki page
  4. Lütke-Eversloh, T & Stephanopoulos, G (2005) Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants. Appl. Environ. Microbiol. 71 7224-8 PubMed EcoliWiki page

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