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Category:Strain:S26 R1d

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Name

S26R1d

Synonyms
Genotype

Hfr (PO2A) fhuA22 phoA4(Am) glnV33(AS) ompF627(T2R) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510

Phenotype

phosphatase positive

Plasmids

Hfr

Phage

λ+

Sources

CGSC:2617
LMBP 4979

Construction

Strain S26R1d is a spontaneous isolate of strain S26

References

PMID:13896933[1] PMID:5327650[2] PMID:4568763[3] PMID:4564480[4] PMID:4286570[5]


Notes

This strain carries the S26 amber mutation (TAG) in the phosphatase gene.

This strain carries the supE suppressor (inserts glutamine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

References

See Help:References for how to manage references in EcoliWiki.

  1. GAREN, A & SIDDIQI, O (1962) Suppression of mutations in the alkaline phosphatase structural cistron of E. coli. Proc. Natl. Acad. Sci. U.S.A. 48 1121-7 PubMed EcoliWiki page
  2. Garen, A et al. (1965) Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J. Mol. Biol. 14 167-78 PubMed EcoliWiki page
  3. Bachmann, BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36 525-57 PubMed EcoliWiki page
  4. Remaut, E & Fiers, W (1972) Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J. Mol. Biol. 71 243-61 PubMed EcoliWiki page
  5. Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed EcoliWiki page

Pages in category "Strain:S26 R1d"

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