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Category:Strain:S26 R1e

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See Help:Strains for more information about strains in EcoliWiki.

Name

S26 R1e

Synonyms

K37

Genotype

Hfr (PO2A) garB10 fhuA22 phoA4(Am) ompF627(T2R) serU132(AS) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510

Phenotype

phosphatase positive

Plasmids

Hfr

Phage

λ+

Sources

CGSC:2598
LMBP 4978

Construction

Strain S26 R1e is a spontaneous isolate of strain S26

References

PMID:5327650[1] PMID:4564480[2] PMID:14337506[3]


Notes

This strain carries the S26 amber mutation (TAG) in the phosphatase gene.

This strain carries the supD suppressor (inserts serine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

References

See Help:References for how to manage references in EcoliWiki.

  1. Garen, A et al. (1965) Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J. Mol. Biol. 14 167-78 PubMed EcoliWiki page
  2. Remaut, E & Fiers, W (1972) Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J. Mol. Biol. 71 243-61 PubMed EcoliWiki page
  3. WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed EcoliWiki page

Pages in category "Strain:S26 R1e"

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