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Category:Strain:ZYCY10P3S2T

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See Help:Strains for more information about strains in EcoliWiki.

Name
Synonyms
Genotype

Cp8.araE ΔendA bla.lacY A177C 3BAD.I-SceI 2BAD.ΦC31 4BAD.Φ31 4BAD.Φ31

Phenotype
Plasmids
Phage
Sources

SBI System Biosciences

Construction
References



Notes

See Help:Strains for more information about strains in EcoliWiki.


araE driven by cp8 promoter [1]
lacY A177C: driven by bla promoter, L-arabinose transporter function [2]
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase


For minicircle plasmid preparation based on attB/attP-Φ31 recombination Parental background plasmid was removed by I-SceI endonuclease

BW27783

References

See Help:References for how to manage references in EcoliWiki.

  1. Khlebnikov, A et al. (2001) Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology (Reading, Engl.) 147 3241-7 PubMed EcoliWiki page
  2. Yu, D et al. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97 5978-83 PubMed EcoliWiki page

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