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DH10B

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See Help:Strains for more information about strains in EcoliWiki.


Alternate Names

Sources

Chromosomal Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[1]

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Comments

useful for generating genomic libraries containing methylated cytosine or adenine residues; useful for plasmid rescue procedures (Copied from GIBCO)

The sequence of DH10B was published in 2008[2] .

See Also

References

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  1. Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed EcoliWiki page
  2. Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed EcoliWiki page