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DH10B

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Alternate Names

The sequence of DH10B was published in 2008[1] .

DH10B

Sources

Chromosomal Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[2]

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Comments

host for pUC and other α-complementation vectors; pBR322
useful for generating genomic libraries containing methylated cytosine or adenine residues
useful for plasmid rescue procedures
Copied from GIBCO

See Also

References

See Help:References for how to manage references in EcoliWiki.
  1. Durfee T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J Bacteriol 190: 2597-606 PubMed EcoliWiki page
  2. Grant SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci U S A 87: 4645-9 PubMed EcoliWiki page
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