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EZ-Tn5 pMOD-5

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At a Glance

pMOD-5, Image made with PlasMapper

Type

Cloning

Origin of Replication

R6Kγori

Copy #
Markers

Ampicillin

Link to Sequence

Epicentre

Features

colE1 origin of replication was eliminated to keep the uncut pMOD vector from contaminating the the transposon constructed. pMOD-5 can be used for a variety of rescue cloning applications.

Accession Number




Notes

History

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Sources

Epicentre

References

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Sequence

You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.

 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
  61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 
 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 
 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 
 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 
 301 TACGACAGCT GTCTCTTATA CACATCTCAA CCATCATCGA TGAATTTTCT CGGGTGTTCT 
 361 CGCATATTGG CTCGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC 
 421 ATGCAAGCTT TAAAAGCCTT ATATATTCTT TTTTTTCTTA TAAAACTTAA AACCTTAGAG 
 481 GCTATTTAAG TTGCTGATTT ATATTAATTT TATTGTTCAA ACATGAGAGC TTAGTACGTG 
 541 AAACATGAGA GCTTAGTACG TTAGCCATGA GAGCTTAGTA CGTTAGCCAT GAGGGTTTAG 
 601 TTCGTTAAAC ATGAGAGCTT AGTACGTTAA ACATGAGAGC TTAGTACGTG AAACATGAGA 
 661 GCTTAGTACG TACTATCAAC AGGTTGAACT GCCAACGACT ACGCACTAGC CAACAAGAGC 
 721 TTCAGGGTTG AGATGTGTAT AAGAGACAGC TGTCTTAATG AATCGGCCAA CGCGCGGGGA 
 781 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
 841 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
 901 AATCAGGGGA TAACGCAGGA AAGAACATGA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 
 961 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 
1021 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 
1081 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 
1141 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 
1201 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 
1261 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 
1321 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 
1381 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 
1441 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 
1501 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 
1561 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 
1621 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 
1681 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 
1741 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 
1801 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 
1861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
1921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
1981 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG 
2041 CCCTTTCGTC