Genes not mapped to a genome sequence
From EcoliWiki
This page has a list of genes that are described in the 1998 linkage map for E. coli K-12 (Edition 10)[1] but have not been correlated with a gene in EcoliWiki. In most cases, the alleles that led to assignment of these gene names are likely to affect known genes, but which gene has not been determined.
| Gene | Approx. Map Location | Mnemonic | Paper(s) describine gene | Notes | Candidate gene(s) |
|---|---|---|---|---|---|
|
abpS |
63.5 |
Arg binding protein |
Low-affinity transport system for arginine and ornithine; periplasmic binding protein CGSC | ||
|
abs |
94.1 |
Antibiotic sensitivity |
Sensitivity and permeability to antibiotics and dyes CGSC | ||
|
acpX |
43.9 |
Acyl carrier protein |
acpS; originally thought to be holo-ACP synthase; perhaps cryptic second gene or regulator CGSC | ||
|
acrC |
4.5 |
Acridine |
Sensitivity to acriflavine; transmembrane protein CGSC | ||
|
adhB |
19.1 |
Alcohol dehydrogenase |
Alcohol dehydrogenase CGSC | ||
|
aga |
70.7 |
Acetylgalactosamine |
Cluster of putative N-acetylgalactosamine pathway genes, including the kba gene, and mannose permease homologs CGSC | ||
|
aroI |
84.2 |
Aromatic |
Function unknown CGSC | ||
|
azaA |
44.6 |
Azaserine |
Mutants azaserine resistant CGSC | ||
|
azaB |
71.9 |
Azaserine |
Mutants azaserine resistant CGSC | ||
|
azl |
58.1 |
Azaleucine |
Mutants azaleucine resistant; regulates ilv and leu CGSC | ||
|
bfm |
85.9 |
BF23 multiplication |
Controls phage BF23 multiplication CGSC | ||
|
bglT |
84.9 |
-Glucoside |
bglE; regulatory gene for BglA CGSC | ||
|
bioP |
86.6 |
Biotin |
bir, birB; biotin transport CGSC | ||
|
brnR |
8.5 |
Branched chain |
Mutants valine resistant, glycylvaline sensitive CGSC | ||
|
brnS |
1.2 |
Branched chain |
Mutants valine resistant, glycylvaline sensitive CGSC | ||
|
brnT |
65.2 |
Branched chain |
Low-affinity transport of Ile CGSC | ||
|
bymA |
93.2 |
Bypass maltose |
Growth on maltose in MalT cells CGSC | ||
|
calA |
95.0 |
Calcium |
Calcium-proton antiport activity CGSC | ||
|
calC |
15.2 |
Calcium |
Calcium transport; mutants defective in chemotaxis CGSC | ||
|
calD |
9.4 |
Calcium |
Calcium transport; mutants defective in chemotaxis CGSC | ||
|
cdsS |
71.7 |
CDP diglyceride synthase |
Stability of CDP diglyceride synthase CGSC | ||
|
cpsA |
45.4 |
Capsular polysaccharide synthesis |
Colanic acid (CPS) biosynthesis CGSC | ||
|
cpsC |
45.8 |
Capsular polysaccharide synthesis |
Colanic acid (CPS) biosynthesis CGSC | ||
|
cpsD |
45.8 |
Capsular polysaccharide synthesis |
Colanic acid (CPS) biosynthesis CGSC | ||
|
cpsE |
45.8 |
Capsular polysaccharide synthesis |
Colanic acid (CPS) biosynthesis CGSC | ||
|
cpsF |
90.2 |
Capsular polysaccharide synthesis |
Colanic acid (CPS) biosynthesis CGSC | ||
|
cpxB |
41.3 |
Conjugative plasmid expression |
Phage Q resistance, membrane protein CGSC | ||
|
crg |
28.8 |
Cold resistant growth |
Allows cold-resistant growth CGSC | ||
|
csiA |
85.3 |
Carbon starvation induced |
Stationary phase inducible protein CGSC | ||
|
csiB |
17.4 |
Carbon starvation induced |
Stationary phase inducible protein CGSC | ||
|
csiC |
66.0 |
Carbon starvation induced |
Stationary phase inducible protein CGSC | ||
|
csiF |
8.6 |
Carbon starvation induced |
Stationary phase inducible protein CGSC | ||
|
cup |
97.0 |
Carbohydrate uptake |
Mutants have defective carbohydrate uptake CGSC | ||
|
cxm |
6.3 |
Carbon-xylose metabolism |
cxr; methyl glyoxal synthesis; D-xylose utilization CGSC | ||
|
cysX |
81.5 |
Cysteine |
Reading frame in opposite orientation within cysE gene; polypeptide synthesized in maxicells CGSC | ||
|
dadQ |
98.9 |
D-Amino acid dehydrogenase |
alnR; regulator of dad regulon CGSC | ||
|
dctB |
16.4 |
Dicarboxylic acid transport |
Uptake of C-4 dicarboxylic acids; 3-fluoromalate resistance, D-tartrate resistant CGSC | ||
|
del |
64.3 |
Deletion |
Affects frequency of IS1-mediated deletions; 1,000-fold reduction in deletion frequency CGSC | ||
|
dgkR |
93.7 |
Diglyceride kinase |
Regulatory CGSC | ||
|
dinY |
41.9 |
Damage inducible |
Repair gene CGSC | ||
|
dnaI |
40.3 |
DNA |
DNA biosynthesis CGSC | ||
|
dppG |
14.0 |
Dipeptide permease |
Uptake of dipeptides; dipeptide permease CGSC | ||
|
dvl |
7.4 |
Dye-visible light? |
Sensitivity to SDS and toluidine blue plus light CGSC | ||
|
ebgB |
69.5 |
Evolved -galactosidase |
Cryptic -galactoside utilization, possible paralog of lacY CGSC | ||
|
envN |
4.2 |
Envelope |
Affects envelope; defects osmotically remedied CGSC | ||
|
envP |
90.4 |
Envelope |
Affects envelope; defects osmotically remedied CGSC | ||
|
envQ |
60.7 |
Envelope |
Affects envelope; defects osmotically remedied CGSC | ||
|
envT |
14.2 |
Envelope |
Affects envelope; defects osmotically remedied CGSC | ||
|
epp |
E-pentapeptide |
Minigene within 23S rRNA encoding functional pentapeptide; erythromycin resistance CGSC | |||
|
esp |
17.3 |
Efficiency site for phage |
Site for efficient packaging of phage T1 CGSC | ||
|
exbC |
61.5 |
Export |
Uptake of enterochelin; resistance or sensitivity to colicins CGSC | ||
|
expA |
22.2 |
Export |
Expression of a group of export proteins CGSC | ||
|
fatA |
71.7 |
Fatty acid |
Utilization of trans unsaturated fatty acids CGSC | ||
|
fcsA |
86.9 |
Filamentous, cold sensitive |
Cold-sensitive cell division mutant CGSC | ||
|
fexB |
86.7 |
F exclusion |
Affects ArcA phenotype CGSC | ||
|
fhlB |
95.1 |
Formate hydrogen-lyase |
Formate hydrogen-lyase; activated by fhlA CGSC | ||
|
fipB |
86.0 |
F1 phage |
Morphogenesis of phage F1 CGSC | ||
|
fipC |
75.0 |
F1 phage |
Morphogenesis of phage F1 CGSC | ||
|
flkB |
95.4 |
FKBP-like |
Periplasmic PPIase, of FK506-binding protein type (EC 5.2.1.8); contains pipecolic acid residue CGSC | ||
|
gadR |
79.6 |
Glutamate deCase |
Regulatory gene for gat? CGSC | ||
|
garA |
15.8 |
Glucarate |
D-Glucarate utilization CGSC | ||
|
garB |
3.6 |
Glucarate |
D-Glucarate utilization CGSC | ||
|
glmX |
26.2 |
Glucosamine |
Affects suppression of glmS mutations by nagB CGSC | ||
|
gltE |
81.4 |
Glutamate |
Glutamyl-tRNA synthetase; possible regulatory subunit CGSC | ||
|
gltH |
21.8 |
Glutamate |
Growth on glutamate CGSC | ||
|
gltR |
92.3 |
Glutamate |
Growth on glutamate at 42C CGSC | ||
|
gprA |
0.3 |
Growth of phage, replication |
Replication of certain lambdoid phage CGSC | ||
|
gprB |
0.2 |
Growth of phage, replication |
Replication of certain lambdoid phage CGSC | ||
|
gurC |
18.0 |
Glucuronide |
Utilization of glucuronides CGSC | ||
|
het |
84.6 |
Heterogeneous size |
cop; possibly structural gene for DNA-binding protein; near ori CGSC | ||
|
hslC |
19.9 |
Heat shock locus |
Protein expressed as heat shock regulon member CGSC | ||
|
hslD |
24.1 |
Heat shock locus |
Protein expressed as heat shock regulon member CGSC | ||
|
hslK |
40.7 |
Heat shock locus |
Protein expressed as heat shock regulon member CGSC | ||
|
hslW |
94.2 |
Heat shock locus |
Heat-inducible; regulatory gene, near groE CGSC | ||
|
ilvF |
57.0 |
Isoleucine-valine (requirement) |
Production of valine-resistant acetolactate synthase activity CGSC | ||
|
ilvR |
99.9 |
Isoleucine-valine | |||
|
ilvU |
6.4 |
Isoleucine-valine (requirement) |
Regulation of ileS and modification of Ile tRNA2 and Val tRNA2 CGSC | ||
|
inm |
79.0 |
Insensitive NG mutagenesis |
Susceptibility to mutagenesis by nitrosoguanidine CGSC | ||
|
isfA |
86.0 |
Inhibits SOS function |
Regulatory gene; SOS-related CGSC | ||
|
ksgB |
37.7 |
Kasugamycin |
High-level resistance to kasugamycin CGSC | ||
|
ksgC |
12.0 |
Kasugamycin |
Resistance to kasugamycin; affects ribosomal protein S2 CGSC | ||
|
ksgD |
30.9 |
Kasugamycin |
Resistance to kasugamycin CGSC | ||
|
leuJ |
13.7 |
Leucine (biosynthesis) |
flr; regulation of leu and ilv operons CGSC | ||
|
leuR |
79.3 |
Leucine |
Regulates level of leucyl-tRNA synthetase CGSC | ||
|
leuY |
9.5 |
Leucine |
Regulates level of leucyl-tRNA synthetase CGSC | ||
|
lev |
9.0 |
Levallorphan |
Resistance to levallorphan CGSC | ||
|
linB |
29.3 |
Lincomycin |
High-level lincomycin resistance CGSC | ||
|
lpcB |
68.0 |
Lipopolysaccharide core |
mrc, pon; T-phage resistance, novobiocin sensitivity CGSC | ||
|
lrb |
7.3 |
L-Ribose (utilization) |
Affects NADPH-linked L-ribose reductase activity CGSC | ||
|
lysX |
63.3 |
Lysine |
Lysine excretion CGSC | ||
|
lytA |
60.6 |
Lytic |
Tolerance to -lactams; autolysis CGSC | ||
|
mafA |
0.9 |
Maintenance of F |
Maintenance of F-like plasmids CGSC | ||
|
mafB |
1.9 |
Maintenance of F |
Maintenance of F-like plasmids CGSC | ||
|
mbrB |
88.6 |
Mothball resistant |
Resistance to camphor vapors; coupling of cell division and replication, growth rate and partitioning CGSC | ||
|
meb |
78.7 |
malE bypass |
Suppressor of malE secB-defective transport of mal-binding protein CGSC | ||
|
mglR |
16.9 |
Methyl-galactoside |
R-MG; regulatory gene CGSC | ||
|
mng |
40.0 |
Manganese |
Manganese resistance CGSC | ||
|
mraA |
2.0 |
Murein cluster a |
D-Alanine carboxypeptidase CGSC | ||
|
mul |
83.1 |
Mutability lambda |
Mutability in UV-radiated lambda phage CGSC | ||
|
murH |
99.3 |
Murein |
Terminal stage in peptidoglycan synthesis, incorporating disaccharide peptide units into wall CGSC | ||
|
mutG |
43.7 |
Mutator |
Mutation causes high C-to-T mutation in second C of CCAGG; near but distinct from vsr; provisionally termed mutG CGSC | ||
|
nalB |
60.2 |
Nalidixic acid |
Sensitivity to nalidixic acid (NAL) CGSC | ||
|
neaB |
75.0 |
Neamine |
Neamine sensitivity CGSC | ||
|
nfnA |
80.8 |
Nitrofurantoin |
Nitrofurantoin sensitivity CGSC | ||
|
nfrD |
54.2 |
N4 (phage) resistant |
Phage N4 susceptibility CGSC | ||
|
non |
45.7 |
Nonmucoid |
Affects capsule formation CGSC | ||
|
oppE |
98.9 |
Oligopeptide permease |
Oligopeptide transport CGSC | ||
|
opr |
19.0 |
rpo reversed |
Rate of degradation of aberrant RNAP-subunit proteins CGSC | ||
|
ops |
66.1 |
Overproduction of polysaccharide |
Level of exopolysaccharide production CGSC | ||
|
oriJ |
30.5 |
Origin of replication |
Locus in defective prophage rac CGSC | ||
|
pac |
31.0 |
Phenylacetate |
Phenylacetate degradation CGSC | ||
|
phxB |
17.0 |
Phage X |
Adsorption of X154 CGSC | ||
|
poaR |
65.9 |
Proline oxidase |
Regulation of proline oxidase production CGSC | ||
|
popD |
0.2 |
Porphyrin |
5-Aminolevulinate dehydratase (EC 4.2.1.24) CGSC | ||
|
prlZ |
71.4 |
Protein localization |
Suppresses export defects in signal sequence mutations CGSC | ||
|
proT |
83.8 |
Proline |
Proline transport carrier protein, putative CGSC | ||
|
pus |
20.6 |
Reverse of sup (amber) |
Reverses accentuation effects of amber suppressor on relB mutations CGSC | ||
|
qin |
35.1 |
Q independent |
kim; cryptic lambdoid phage CGSC | ||
|
qmeC |
75.3 |
Q membrane |
Glycine resistance; penicillin sensitivity; membrane defect CGSC | ||
|
qmeD |
64.4 |
Q membrane |
Glycine resistance; penicillin sensitivity; membrane defect CGSC | ||
|
qmeE |
37.9 |
Q membrane |
Glycine resistance; penicillin sensitivity; membrane defect CGSC | ||
|
ras |
9.9 |
Radiation sensitive |
Sensitive to X rays and UV CGSC | ||
|
rdgA |
16.1 |
RecA-dependent growth |
Dependence of growth upon recA gene product CGSC | ||
|
relX |
62.8 |
Relaxed |
Control of ppGpp synthesis CGSC | ||
|
rer |
89.9 |
Resistance to radiation |
Resistance to UV and gamma radiation CGSC | ||
|
ridA |
73.1 |
Rifampin dependence |
Rifampin resistance and dependence CGSC | ||
|
ridB |
85.5 |
Rifampin dependence |
Transcription and translation; rifampin (rifamycin) sensitivity CGSC |
rho | |
|
rimB |
38.9 |
Ribosomal modification |
50S ribosomal subunit maturation CGSC | ||
|
rimC |
26.2 |
Ribosomal modification |
50S ribosomal subunit maturation CGSC | ||
|
rimD |
87.7 |
Ribosomal modification |
50S ribosomal subunit maturation CGSC | ||
|
rimE |
74.1 |
Ribosomal modification |
Ribosomal protein modification CGSC | ||
|
rimH |
13.6 |
Ribosomal modification |
stsB; ribosomal modification CGSC | ||
|
rit |
89.2 |
Ribosomal thermolability |
Affects thermolability of 50S ribosomal subunit CGSC | ||
|
rorB |
85.1 |
Roentgen resistance |
Sensitivity to ionizing radiation, mitomycin C CGSC | ||
|
sds |
30.4 |
Suppresses disulfide bond |
Motility defects CGSC | ||
|
semA |
40.5 |
Sensitivity to microcin |
Sensitivity to microcin E492 CGSC | ||
|
serR |
2.3 |
Serine |
Regulates level of seryl-tRNA synthetase CGSC | ||
|
sfiC |
25.8 |
Septum formation inhibition |
Element of e14, inhibitor of cell division CGSC | ||
|
sipC |
82.6 |
Suppressor of increased permeability |
Mutations reverse the susceptibility to vancomycin and other hydrophobic antibiotics caused by TraT plasmid gene CGSC | ||
|
sipD |
82.7 |
Suppressor of increased permeability |
Same phenotype as that produced by sipC CGSC | ||
|
sir |
61.2 |
SOS-independent repair |
SOS-independent repair of mitomycin C-induced DNA damage CGSC | ||
|
sloB |
75.0 |
Slow growth |
Tolerance for amidinopenicillin and nalidixic acid; slow growth rate CGSC | ||
|
slrR |
60.9 |
Sorbitol |
gutR; regulatory gene for srl CGSC | ||
|
srnA |
9.4 |
Stable RNA |
Degradation of stable RNA CGSC | ||
|
ssaE |
52.5 |
Suppression secA |
Suppresses secA mutations CGSC | ||
|
ssaG |
41.8 |
Suppression secA |
Suppresses secA mutations CGSC | ||
|
ssaH |
94.1 |
Suppression secA |
Suppresses secA mutations CGSC | ||
|
ssyD |
3.0 |
Suppression of secY |
Suppressor of secY mutation CGSC | ||
|
stfZ |
2.3 |
fts in reverse |
Antisense RNA blocks ftsZ mRNA translation, inhibits cell division CGSC | ||
|
stkA |
77.5 |
Suppressor of transposase killing |
Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC | ||
|
stkB |
86.1 |
Suppressor of transposase killing |
Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC | ||
|
stkC |
98.5 |
Suppressor of transposase killing |
Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC | ||
|
stkD |
28.6 |
Suppressor of transposase killing |
Suppresses cell aberrations and death caused by Tn5 transposase overexpression CGSC | ||
|
strC |
7.0 |
Streptomycin |
strB; low-level streptomycin resistance; modifies ribosome structure CGSC | ||
|
strM |
78.3 |
Streptomycin |
asuF?; control of ribosomal ambiguity CGSC | ||
|
stsA |
84.7 |
Altered RNase activity CGSC | |||
|
suhA |
78.4 |
Suppressor heat-shock proteins |
Induction of heat shock genes CGSC | ||
|
tabC |
86.3 |
T4 abortion |
Mutants fail to support growth of T4 CGSC | ||
|
tanA |
41.6 |
Trehalose, anaerobic |
Anaerobic growth on trehalose CGSC | ||
|
tanB |
68.1 |
Trehalose, anaerobic |
Anaerobic growth on trehalose CGSC | ||
|
tdi |
4.3 |
Transduction inhibition |
Affects transduction, transformation, and rates of mutation CGSC | ||
|
thdA |
10.5 |
Thiophene degradation |
Degradation of furans and thiophenes; may be tlnA? CGSC | ||
|
psu |
1.3 min |
polarity suppressor | |||
|
thdC |
94.2 |
Thiophene degradation |
Degradation of furans and thiophenes CGSC | ||
|
thdD |
99.8 |
Thiophene degradation |
Degradation of furans and thiophenes CGSC | ||
|
ackB |
39 min |
acetate kinase |
Ack- mutants were defective in aerobic growth on acetate or glucose as well as acetate accumulation from glucose & mapped near 39' (ackA mapped to ~50'). PMID:7033467[6] CGSC | ||
|
tlnA |
10.5 |
Thiolutin |
tlnI; resistance or sensitivity to thiolutin; may be thdA? CGSC | ||
|
alaB |
alanine-glutamate transaminase |
alaB was found to be capable of multi-copy suppression of an alanine auxotrophy. PMID:2890623[7] | |||
|
tnm |
92.0 |
Tn migration |
Transposition of Tn9 and other transposons, development of phage Mu CGSC | ||
|
dgd |
D-galactose dehydrogenase | ||||
|
tolD |
22.9 |
Tolerance |
Bacteriocin tolerant; tolerance to colicins E2, E3, and ampicillin CGSC | ||
|
treE |
catabolic trehalose-6-phosphate phosphatase |
In the absence of osmotic stress, trehalose induces converstion to free glucose and a glucose polymer, in a pathway involving TreE. PMID:8391102[8] | |||
|
tolE |
22.9 |
Tolerance |
Bacteriocin tolerant; tolerance to colicins E2, E3, and ampicillin CGSC | ||
|
tolI |
0.1 |
Tolerance |
Bacteriocin tolerant; sensitivity to colicins Ia and Ib CGSC | ||
|
tolJ |
0.1 |
Tolerance |
Bacteriocin tolerant; sensitivity to L, A, S4, E, and K CGSC | ||
|
tsmA |
40.1 |
Thymine suppression modifier |
Affects suppression efficiency for nonsense and frameshift mutations of Thy strains CGSC | ||
|
ups |
27.1 |
Up-suppression? |
Efficiency of nonsense suppressors; see prfA PMID:6265322[9] CGSC | ||
|
uvh |
90.2 |
UV hyperresistant to UVC |
Resistance to UVC, peroxide and antibiotics; may affect SOS repair PMID:8988611[10] CGSC | ||
|
uvs |
91.1 |
UV sensitivity |
UV-sensitive mutants, locus linked to uvr PMID:8988611[10] CGSC |
| |
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Berlyn MK (1998) Linkage map of Escherichia coli K-12, edition 10: the traditional map. Microbiol Mol Biol Rev 62: 814-984 PubMed EcoliWiki page
- ↑ 2.0 2.1 Guardiola J et al. (1974) Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J Bacteriol 117: 393-405 PubMed EcoliWiki page
- ↑ Wada C et al. (1977) Replication of Fpoh+ plasmid in mafA mutants of Escherichia coli defective in plasmid maintenance. Mol Gen Genet 152: 211-7 PubMed EcoliWiki page
- ↑ 4.0 4.1 Wada C & Yura T (1979) Escherichia coli mutants incapable of supporting replication of F-like plasmids at high temperature: isolation and characterization of mafA and mafB mutants. J Bacteriol 140: 864-73 PubMed EcoliWiki page
- ↑ Ephrati-Elizur E (1993) A mutation in a new gene of Escherichia coli, psu, requires secondary mutations for survival: psu mutants express a pleiotropic suppressor phenotype. J Bacteriol 175: 207-13 PubMed EcoliWiki page
- ↑ Pascal MC et al. (1981) Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism. J Gen Microbiol 124: 35-42 PubMed EcoliWiki page
- ↑ Wang MD et al. (1987) Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids. J Bacteriol 169: 5610-4 PubMed EcoliWiki page
- ↑ Strøm AR & Kaasen I (1993) Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol Microbiol 8: 205-10 PubMed EcoliWiki page
- ↑ Cowman A & Beacham IR (1980) Molecular cloning of the gene (ush) from Escherichia coli specifying periplasmic UDP-sugar hydrolase (5'-nucleotidase). Gene 12: 281-6 PubMed EcoliWiki page
- ↑ 10.0 10.1 Ahmad SI (1996) A mutant of Escherichia coli hyper-resistant to a number of DNA damaging agents: location of the mutational site. J Photochem Photobiol B 36: 47-53 PubMed EcoliWiki page
