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Hi-Control(tm) 10G

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Alternate Names

Sources

Chromosomal Genotype

mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA

Plasmids

Mini-F lacIq1(Gentr)

Phage

Phenotypes

Construction

Notes

  • The HI-Control 10G cells contain a single-copy BAC plasmid harboring a specially engineered version of the lacIq1 repressor allele. The lacIq1 allele expresses ~170-fold more lac repressor protein than the wild-type lacI gene.
  • For stable cloning of T7 protein expression plasmids.
  • Resistant to phage T1.


References

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