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K12ΔH1Δtrp

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Name K12ΔH1Δtrp
Synonyms K12deltaH1deltatrp
Genotype lacZam Δ(bio-uvrB) Δ(trpE-A)2 rpsL Δ(Nam7Nam53 cI857 ΔH1)
Phenotype streptomycin resistant
Sources LMBP 69
References PMID:372049 PMID:6271633

Notes

Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. cI857 is ind-.