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Category:Strain:MC1061

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See Help:Strains for more information about strains in EcoliWiki.

Name

MC1061

Synonyms
Genotype

araD139 Del(araA-leu)7697 Del(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2

This is the genotype at CGSC as of Nov, 2008. Note that there are differences with the original genotype [1]

Phenotype

streptomycin resistance

Plasmids
Phage
Sources

CGSC
LMBP 472

Construction

Made by Malcolm Casadaban

References

PMID:6997493[1]


Notes

Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification.
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.
The zeocin resistance is most probably related to the presence of a functional recA gene (recA+).

References

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  1. 1.0 1.1 Casadaban, MJ & Cohen, SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138 179-207 PubMed EcoliWiki page

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