PortEco logo

MG1655 seqA-mEOS3.2

From EcoliWiki
Jump to: navigation, search
Name MG1655 seqA-mEOS3.2
Synonyms
Genotype F λ ilvG rfb-50 rph-1 seqA-mEOS3.2:kmR
Phenotype Kanamycin resistant
Sources LMBP 9777
References PMID:26449690

Notes

The native seqA gene was fused at its 3' end to the fluorescent protein gene mEOS3.2, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.