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Methods:Acetone Protein Precipitation

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Contents

Purpose

Protein concentration Share your knowledge and ideas. How you can help. See Help pages if you need help with using the wiki

Materials Needed

Ice cold Acetone

Sample

Ice bucket

Centrifuge/Microcentrifuge

Protocol

PROCEDURE:

1. Mix sample with at least two volumes of ice cold Acetone

2. Place on ice (or in –20C) for minimum 1 hour

3. Centrifuge at 13,000 rpm (4C) for 5 minutes (length of spin depends on sample volume)

4. Remove supernatant materials & save on ice

5. Rinse pellet with ice cold acetone

6. Centrifuge at 13,000 rpm (4C) for 5 minutes (length of spin depends on sample volume)

7. Remove supernatant materials and discard

8. Air dry the pellet

9. Resuspend in buffer of choice

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References

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