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- Determine total protein concentration of a sample
- Standard curve using BSA
- Sample of sufficient concentration
- Bradford reagent (see Pierce or Bio-Radfor a commercial kit or the original paper by Bradford)
- Spectrophotometer capable of reading at 595nm
- Bradford assays measure TOTAL protein concentration (any contaminating protein will be measured along with your desired protein)
- Bradford assays work for ug/mL concentrations between the ranges of 1 - >1000 ug/mL total protein
- Bradford assays are used for proteins of greater than 3kDa
- Bradford assays are generally sensitive to detergent, but a detergent-compatible kit is available from Bio-Rad
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