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Methods:DNA Precipitation

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Contents

Purpose

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Materials Needed

Protocol

  1. Add 1/10 volume 3M NaOAc (pH 5.2) and 2 volumes of 100% Ethanol. Invert.
  2. Put at -20oC for at least 30 mins.
  3. Centrifuge at 4oC for 15 min.
  4. Pipet off supernatant. Add 500μL ICE-COLD 70% ethanol.
  5. Centrifuge at room temp for 5 mins.
  6. Pipet off supernatant & allow to air dry.

Usage Examples

User notes

  • If you want, you can add 3μL of glycogen to help you see a pellet.

Related GO Terms

References

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