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Methods:FISH

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Contents

Purpose

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FISH, or Fluorescence In Situ Hybridization, is a microscope-driven technique used to locate DNA foci inside cells[1][2]

Materials Needed

Protocol

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References

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  1. Li, Y et al. (2002) The segregation of the Escherichia coli origin and terminus of replication. Mol. Microbiol. 46 985-96 PubMed EcoliWiki page
  2. Bates, D & Kleckner, N (2005) Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation. Cell 121 899-911 PubMed EcoliWiki page