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Methods:Glycerol Freezer Stock

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Contents

Purpose

To permanently store E.coli strains

Materials Needed

50% glycerol overnight culture -80C freezer

Protocol

  • Inoculate 2 ml LB + antibiotic with a single colony
  • Incubate shaking overnight at 37C (temperature may be different)
  • In a freezer tube, add
    • 450μl 50% glycerol
    • 1350μl culture
  • Vortex
  • Freeze in liquid nitrogen (optional)
  • Store at -80C

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References

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