Methods:Glycerol Freezer Stock
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To permanently store E.coli strains
50% glycerol overnight culture -80C freezer
- Inoculate 2 ml LB + antibiotic with a single colony
- Incubate shaking overnight at 37C (temperature may be different)
- In a freezer tube, add
- 450μl 50% glycerol
- 1350μl culture
- Freeze in liquid nitrogen (optional)
- Store at -80C
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