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LB is a commonly used rich medium. LB originally indicated Lysogeny Broth, but currently is referred to as Luria-Bertani medium.


per liter

  • 10 g Bacto-tryptone
  • 5 g yeast extract
  • 10 g NaCl

premixed LB is available from many commercial sources

Autoclave to sterilize.

User notes



  1. Sezonov, G et al. (2007) Escherichia coli physiology in Luria-Bertani broth. J. Bacteriol. 189 8746-9 PubMed EcoliWiki page

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