PortEco logo

Methods:Minimal medium 132

From EcoliWiki
Jump to: navigation, search

You can help EcoliWiki by editing the content of this page. For information about becoming a registered user and obtaining editing privileges, see Help:Accounts.

Contents

Purpose

Share your knowledge and ideas. How you can help. See Help pages if you need help with using the wiki

Materials Needed

3 g KH2PO4

7 g K2HPO4

1 g (NH4)2SO4

0.1 g MgSO4.7H20

10-6 M MnSO4

10-6 M Fe citrate

1 liter H2O

Protocol

Don't add the carbon source or other supplements until after the salts mixture has been autoclaved.

Sterile glucose or glycerol were added to sterile minimal medium 132 to a final concentration of 0.5%. [1]

Links

User notes

References

See Help:References for how to manage references in EcoliWiki. [1]

  1. 1.0 1.1 GLANSDORFF, N (1965) TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. Genetics 51 167-79 PubMed EcoliWiki page