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Methods:Mitomycin C Sensitivity Assay

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Contents

Purpose

Materials Needed

  • LB Plates containing 0.5µg/ml of mitomycin C (abbreviated MMC)
  • Incubator

Protocol

Taken from Lopez et al. (2005)[1]

  1. Grow culture to mid-logarithmic phase (OD600 = 0.4)
  2. Serially dilute the cultures (1:10 dilutions)
  3. Plate on LB + 0.5µg/ml MMC and on plates w/o antibiotic
  4. Incubate overnight (up to 72 hours)

Other Usage Examples

  • Identifying genes involved in the DNA damage response[2]

User notes

References

See Help:References for how to manage references in EcoliWiki.

  1. Lopez, CR et al. (2005) A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol. Microbiol. 58 80-101 PubMed EcoliWiki page
  2. Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed EcoliWiki page