Methods:UV Sensitivity Test
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This test in usually used to test whether a strain is rec-. Rec- strains cannot repair double stranded break due to UV exposure and therefore die.
- UV transilluminator
- piece of cardboard or other opaque material
- control strains- one rec- and one rec+
Before you begin you may need to determine the amount of UV exposure needed to see a difference in growth. UV transilluminators will vary.
- Streak candidates out for single colonies
- Streak candidates in a straight line from 1 end of the plate to the other along with the controls
- Divide the plate into 4 sections
- Place the cardboard on the transilluminator
- Turn the plate over and shield 3/4 of the plate from UV exposure for 10 seconds
- Move the plate so that only 1/2 of it is shielded for another 10 seconds
- Move the plate so that only 1/4 of it is shielded for another 10 seconds
- Turn off the UV
- Incubate the plate at 37C overnight
- Evaluate growth compared to the controls
See Help:References for how to manage references in EcoliWiki.
- Miller, JH (1972) Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)