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Methods:mRNA isolation by subtractive hybridization

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Contents

Purpose

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Total RNA is mostly rRNA. Removing rRNA improves the yield of mRNA for many molecular biology/transcriptome methods.

Basis

rRNA is hybridized to a DNA or RNA antisense probe. The rRNA is then either physically removed[1], or selectively degraded.

Materials Needed

Protocol

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References

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  1. Su, C & Sordillo, LM (1998) A simple method to enrich mRNA from total prokaryotic RNA. Mol. Biotechnol. 10 83-5 PubMed EcoliWiki page