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OverExpress(tm)C41(DE3) pLysS

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Alternate Names

Sources

Lucigen Corporation

Chromosomal Genotype

F ompT gal dcm hsdSB(rB- mB-)(DE3)

Plasmids

pLysS

Phage

Phenotypes

Construction

Notes

  • The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one uncharacterized mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
  • an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
  • Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lac promoter.
  • The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.


Derivatives

Category:Expression Strain


References

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