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PMID:15004283

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Contents

Citation

Kumar, JK, Tabor, S and Richardson, CC (2004) Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 101:3759-64

Abstract

Thioredoxin, a ubiquitous and evolutionarily conserved protein, modulates the structure and activity of proteins involved in a spectrum of processes, such as gene expression, apoptosis, and the oxidative stress response. Here, we present a comprehensive analysis of the thioredoxin-linked Escherichia coli proteome by using tandem affinity purification and nanospray microcapillary tandem mass spectrometry. We have identified a total of 80 proteins associated with thioredoxin, implicating the involvement of thioredoxin in at least 26 distinct cellular processes that include transcription regulation, cell division, energy transduction, and several biosynthetic pathways. We also found a number of proteins associated with thioredoxin that either participate directly (SodA, HPI, and AhpC) or have key regulatory functions (Fur and AcnB) in the detoxification of the cell. Transcription factors NusG, OmpR, and RcsB, not considered to be under redox control, are also associated with thioredoxin.

Links

PubMed PMC374317 Online version:10.1073/pnas.0308701101

Keywords

Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; Escherichia coli/metabolism; Magnetic Resonance Spectroscopy; Proteins/chemistry; Proteins/metabolism; Proteome; Thioredoxins/chemistry; Thioredoxins/metabolism

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Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

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