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PMID:15489430
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Van Dyk, TK, Templeton, LJ, Cantera, KA, Sharpe, PL and Sariaslani, FS (2004) Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?J. Bacteriol. 186:7196-204
| Abstract | Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS, encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as inducers of yhcRQP expression. The efflux function encoded by yhcP was proven by the hypersensitivity to pHBA of a yhcP mutant strain. A yhcS mutant strain was also hypersensitive to pHBA. Expression of yhcQ and yhcP was necessary and sufficient for suppression of the pHBA hypersensitivity of the yhcS mutant. Only a few aromatic carboxylic acids of hundreds of diverse compounds tested were defined as substrates of the YhcQP efflux pump. Thus, we propose renaming yhcS, yhcR, yhcQ, and yhcP, to reflect their role in aromatic carboxylic acid efflux, to aaeR, aaeX, aaeA, and aaeB, respectively. The role of pHBA in normal E. coli metabolism and the highly regulated expression of the AaeAB efflux system suggests that the physiological role may be as a "metabolic relief valve" to alleviate toxic effects of imbalanced metabolism. |
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0042221 |
response to chemical stimulus |
IDA: Inferred from Direct Assay |
P |
The authors used DNA microarray analysis to show that expression of the aaeA gene was induced in the presence of p-hydroxybenzoic acid (pHBA) (pp 7198). |
complete | |||
| GO:0006810 |
transport |
IMP: Inferred from Mutant Phenotype |
P |
E. coli aaeB- mutants were greater than 8 times more sensitive to p-hydroxybenzoic acid (pHBA) and other aromatic carboxylic acids (Table 3). |
complete | |||
| GO:0046942 |
carboxylic acid transport |
IGI: Inferred from Genetic Interaction |
EcoliWiki:aaeB |
P |
Zone of growth inhibition assays showed that expression of AaeA is required for restoration of wild-type resistance to p-hydroxybenzoic acid (pHBA) (pp. 7200). |
complete | ||
| GO:0046942 |
carboxylic acid transport |
IGI: Inferred from Genetic Interaction |
EcoliWiki:aaeA |
P |
E. coli aaeB- mutants were greater than 8 times more sensitive to p-hydroxybenzoic acid (pHBA) and other aromatic carboxylic acids (Table 3). |
complete | ||
| GO:0042221 |
response to chemical stimulus |
IGI: Inferred from Genetic Interaction |
EcoliWiki:aaeA |
P |
complete | |||
| GO:0006810 |
transport |
IGI: Inferred from Genetic Interaction |
EcoliWiki:aaeB |
P |
AaeA was shown to be a required component of an efflux pump specific to aromatic carboxylic acids (Table 3). |
complete | ||
| GO:0006350 |
transcription |
IMP: Inferred from Mutant Phenotype |
P |
In bioluminescence response assays, aaeR- mutants were unable to induce expression of aaeABX in the presence of increasing concentrations of p-hydroxybenzoic acid (pHBA) unless aaeR was provided in trans from a plasmid (Table 2). |
complete | |||
| GO:0006355 |
regulation of transcription, DNA-dependent |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0016563 |
transcription activator activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
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