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PMID:17164287

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Contents

Citation

Grainger, DC, Aiba, H, Hurd, D, Browning, DF and Busby, SJ (2007) Transcription factor distribution in Escherichia coli: studies with FNR protein. Nucleic Acids Res. 35:269-78

Abstract

Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5' end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.

Links

PubMed PMC1802558 Online version:10.1093/nar/gkl1023

Keywords

Binding Sites; Chromatin Immunoprecipitation; Chromosomes, Bacterial; DNA, Bacterial/chemistry; DNA, Bacterial/isolation & purification; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/analysis; Escherichia coli Proteins/metabolism; Iron-Sulfur Proteins/analysis; Iron-Sulfur Proteins/metabolism; Oligonucleotide Array Sequence Analysis; Regulatory Elements, Transcriptional; Sequence Analysis, DNA; Transcription Factors/analysis; Transcription Factors/metabolism

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Useful Materials and Methods

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


Data

Strain/Organism Assay Analyte Variable Conditions Notes Links

MG1655

ChIP-chip

FNR

MG1655

ChIP-chip

IHF

MG1655

ChIP-chip

RNAP

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See also

References

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