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PMID:19376873

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Contents

Citation

Handford, JI, Ize, B, Buchanan, G, Butland, GP, Greenblatt, J, Emili, A and Palmer, T (2009) Conserved network of proteins essential for bacterial viability. J. Bacteriol. 191:4732-49

Abstract

The yjeE, yeaZ, and ygjD genes are highly conserved in the genomes of eubacteria, and ygjD orthologs are also found throughout the Archaea and eukaryotes. In this study, we have constructed conditional expression strains for each of these genes in the model organism Escherichia coli K12. We show that each gene is essential for the viability of E. coli under laboratory growth conditions. Growth of the conditional strains under nonpermissive conditions results in dramatic changes in cell ultrastructure. Deliberate repression of the expression of yeaZ results in cells with highly condensed nucleoids, while repression of yjeE and ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA. Each of the three conditional expression strains can be complemented by multicopy clones harboring the rstA gene, which encodes a two-component-system response regulator, strongly suggesting that these proteins are involved in the same essential cellular pathway. The results of bacterial two-hybrid experiments show that YeaZ can interact with both YjeE and YgjD but that YgjD is the preferred interaction partner. The results of in vitro experiments indicate that YeaZ mediates the proteolysis of YgjD, suggesting that YeaZ and YjeE act as regulators to control the activity of this protein. Our results are consistent with these proteins forming a link between DNA metabolism and cell division.

Links

PubMed PMC2715707 Online version:10.1128/JB.00136-09

Keywords

Escherichia coli K12/genetics; Escherichia coli K12/metabolism; Escherichia coli K12/physiology; Escherichia coli K12/ultrastructure; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial/genetics; Gene Expression Regulation, Bacterial/physiology; Genome, Bacterial/genetics; Genome, Bacterial/physiology; Microbial Viability/genetics; Microscopy, Electron, Transmission; Protein Binding; Protein Multimerization; Two-Hybrid System Techniques

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

yeaZ:Gene_Product(s)

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

UniProtKB:P05852
UniProtKB:P0AF67

F

Seeded from EcoCyc (v14.0)

complete

gcp:Gene_Product(s)

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

UniProtKB:P76256

F

Seeded from EcoCyc (v14.0)

complete

yjeE:Gene_Product(s)

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

UniProtKB:P76256

F

Seeded from EcoCyc (v14.0)

complete

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References

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