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PMID:19767843

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Contents

Citation

Moen, B, Janbu, AO, Langsrud, S, Langsrud, O, Hobman, JL, Constantinidou, C, Kohler, A and Rudi, K (2009) Global responses of Escherichia coli to adverse conditions determined by microarrays and FT-IR spectroscopy. Can. J. Microbiol. 55:714-28

Abstract

The global gene expression and biomolecular composition in an Escherichia coli model strain exposed to 10 adverse conditions (sodium chloride, ethanol, glycerol, hydrochloric and acetic acid, sodium hydroxide, heat (46 degrees C), and cold (15 degrees C), as well as ethidium bromide and the disinfectant benzalkonium chloride) were determined using DNA microarrays and Fourier transform infrared (FT-IR) spectroscopy. In total, approximately 40% of all investigated genes (1682/4279 genes) significantly changed expression, compared with a nonstressed control. There were, however, only 3 genes (ygaW (unknown function), rmf (encoding a ribosomal modification factor), and ghrA (encoding a glyoxylate/hydroxypyruvate reductase)) that significantly changed expression under all conditions (not including benzalkonium chloride). The FT-IR analysis showed an increase in unsaturated fatty acids during ethanol and cold exposure, and a decrease during acid and heat exposure. Cold conditions induced changes in the carbohydrate composition of the cell, possibly related to the upregulation of outer membrane genes (glgAP and rcsA). Although some covariance was observed between the 2 data sets, principle component analysis and regression analyses revealed that the gene expression and the biomolecular responses are not well correlated in stressed populations of E. coli, underlining the importance of multiple strategies to begin to understand the effect on the whole cell.

Links

PubMed Online version:10.1139/w09-016

Keywords

Bacterial Proteins/genetics; Escherichia coli/chemistry; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Fatty Acids/metabolism; Gene Expression Regulation, Bacterial; Oligonucleotide Array Sequence Analysis; Organic Chemicals/pharmacology; Spectroscopy, Fourier Transform Infrared; Temperature

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

rmf:Gene_Product(s)

GO:0033554

cellular response to stress

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

ghrA:Gene_Product(s)

GO:0033554

cellular response to stress

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

alaE:Gene_Product(s)

GO:0033554

cellular response to stress

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

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References

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