PortEco logo


From EcoliWiki
Jump to: navigation, search



Hudson, BP, Quispe, J, Lara-González, S, Kim, Y, Berman, HM, Arnold, E, Ebright, RH and Lawson, CL (2009) Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proc. Natl. Acad. Sci. U.S.A. 106:19830-5


We present the experimentally determined 3D structure of an intact activator-dependent transcription initiation complex comprising the Escherichia coli catabolite activator protein (CAP), RNA polymerase holoenzyme (RNAP), and a DNA fragment containing positions -78 to +20 of a Class I CAP-dependent promoter with a CAP site at position -61.5 and a premelted transcription bubble. A 20-A electron microscopy reconstruction was obtained by iterative projection-based matching of single particles visualized in carbon-sandwich negative stain and was fitted using atomic coordinate sets for CAP, RNAP, and DNA. The structure defines the organization of a Class I CAP-RNAP-promoter complex and supports previously proposed interactions of CAP with RNAP alpha subunit C-terminal domain (alphaCTD), interactions of alphaCTD with sigma(70) region 4, interactions of CAP and RNAP with promoter DNA, and phased-DNA-bend-dependent partial wrapping of DNA around the complex. The structure also reveals the positions and shapes of species-specific domains within the RNAP beta', beta, and sigma(70) subunits.


PubMed PMC2775702 Online version:10.1073/pnas.0908782106


Base Sequence; Cyclic AMP Receptor Protein/chemistry; Cyclic AMP Receptor Protein/ultrastructure; DNA, Bacterial/chemistry; DNA, Bacterial/ultrastructure; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/ultrastructure; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/ultrastructure; Macromolecular Substances/chemistry; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Promoter Regions, Genetic; Protein Structure, Tertiary; Protein Subunits/chemistry; Transcription, Genetic


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.


Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also


See Help:References for how to manage references in EcoliWiki.