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Citation

Kim, Y and Wood, TK (2010) Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochem. Biophys. Res. Commun. 391:209-13

Abstract

Persisters are cells which evade stresses like antibiotics and which are characterized by reduced metabolism and a lack of genetic alterations required to achieve this state. We showed previously that MqsR and MqsA of Escherichia coli are a toxin-antitoxin pair that influence cell physiology (e.g., biofilm formation and motility) via RNase activity as well as through regulation of toxin CspD. Here, we show that deletion of the mqsRA locus decreases persister cell formation and, consistent with this result, over production of MqsR increases persister cell formation. Furthermore, toxins Hha, CspD, and HokA increase persister cell formation. In addition, by overproducing MqsR in a series of isogenic mutants, we show that Hha and CspD are necessary for persister cell formation via MqsR overexpression. Surprisingly, Hfq, a small RNA chaperone, decreases persistence. A whole-transcriptome study shows that Hfq induces transport-related genes (opp genes and dppA), outer membrane protein-related genes (ybfM and ybfN), toxins (hha), and proteases (clpX, clpP, and lon). Taken together, these results indicate that toxins CspD, Hha, and HokA influence persister cell formation via MqsR and that Hfq plays an important role in the regulation of persister cell formation via regulation of transport or outer membrane proteins OppA and YbfM.

Links

PubMed PMC2812665 Online version:10.1016/j.bbrc.2009.11.033

Keywords

Anti-Bacterial Agents/pharmacology; Bacterial Toxins/genetics; Bacterial Toxins/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Profiling; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Host Factor 1 Protein/genetics; Host Factor 1 Protein/metabolism; RNA/metabolism; Stress, Physiological

Significance

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The mRNA interferase MqsR of E. coli[1] is significant in that it is only the second toxin that, upon deleting the gene that encodes it, causes persister cell formation to decrease[2]. In addition, the manuscript links for the first time E. coli proteins Hha, CspD, HokA, Hfq, YbfM, and OppA to persistence[2]. The work also provides whole transcriptome data for the influence of Hhq on metabolism[2].

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References

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  1. Brown, BL et al. (2009) Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties. PLoS Pathog. 5 e1000706 PubMed EcoliWiki page
  2. 2.0 2.1 2.2 Kim, Y & Wood, TK (2010) Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochem. Biophys. Res. Commun. 391 209-13 PubMed EcoliWiki page