PortEco logo


From EcoliWiki
Jump to: navigation, search



Domain, F and Levy, SB (2010) GyrA interacts with MarR to reduce repression of the marRAB operon in Escherichia coli. J. Bacteriol. 192:942-8


Bacterial two-hybrid studies of randomly cloned Escherichia coli DNA identified a physical interaction between GyrA, subunit A of gyrase, and MarR, a repressor of the marRAB operon. GyrA-His immobilized on Ni-nitrilotriacetic acid (NiNTA) resin bound MarR, while MarR alone did not bind. GyrA interfered with MarR binding to marO, as detected by electrophoretic mobility assays. In a strain bearing the marRAB operon and a marO-lacZ reporter, overexpression of GyrA increased LacZ activity, indicating decreased repression of marO-lacZ by MarR. These results were confirmed by an increased survival of cells treated with quinolones and other antibiotics when GyrA was overexpressed. This work, like a previous study examining TktA (12), shows that unrelated proteins can regulate MarR activity. The findings reveal an unexpected regulatory function of GyrA in antibiotic resistance.


PubMed PMC2812966 Online version:10.1128/JB.01259-09


Artificial Gene Fusion; DNA Gyrase/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Genes, Reporter; Operon; Protein Binding; Protein Interaction Mapping; Repressor Proteins/metabolism; Two-Hybrid System Techniques; beta-Galactosidase/genetics; beta-Galactosidase/metabolism


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.


Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also


See Help:References for how to manage references in EcoliWiki.