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PMID:19943910

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Contents

Citation

Christensen-Dalsgaard, M, Jørgensen, MG and Gerdes, K (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Mol. Microbiol. 75:333-48

Abstract

Prokaryotic toxin - antitoxin (TA) loci encode mRNA interferases that inhibit translation, either by cleaving mRNA codons at the ribosomal A site or by cleaving any RNA site-specifically. So far, seven mRNA interferases of Escherichia coli have been identified, four of which cleave mRNA by a translation-dependent mechanism. Here, we experimentally confirmed the presence of three novel TA loci in E. coli. We found that the yafNO, higBA (ygjNM) and ygiUT loci encode mRNA interferases related to RelE. YafO and HigB cleaved translated mRNA only, while YgiU cleaved RNA site-specifically at GC[A/U], independently of translation. Thus, YgiU is the first RelE-related mRNA interferase that cleaves mRNA independently of translation, in vivo. All three loci were induced by amino acid starvation, and inhibition of translation although to different degrees. Carbon starvation induced only two of the loci. The yafNO locus was induced by DNA damage, but the transcription originated from the dinB promoter. Thus, our results showed that the different TA loci responded differentially to environmental stresses. Induction of the three loci depended on Lon protease that may sense the environmental stresses and activate TA loci by cleavage of the antitoxins. Transcription of the three TA operons was autoregulated by the antitoxins.

Links

PubMed PMC2814082 Online version:10.1111/j.1365-2958.2009.06969.x

Keywords

Antitoxins/genetics; Antitoxins/pharmacology; Bacterial Toxins/chemistry; Bacterial Toxins/genetics; Bacterial Toxins/toxicity; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/genetics; Colony-Forming Units Assay; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Environment; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Messenger/genetics; Sequence Homology

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

yafN:Gene_Product(s)

GO:0006355

regulation of transcription, DNA-dependent

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

ygiT:Gene_Product(s)

GO:0006355

regulation of transcription, DNA-dependent

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

ygjM:Gene_Product(s)

GO:0006355

regulation of transcription, DNA-dependent

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

ygjN:Gene_Product(s)

GO:0004521

endoribonuclease activity

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

ygjN:Gene_Product(s)

GO:0017148

negative regulation of translation

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

ygjN:Gene_Product(s)

GO:0043488

regulation of mRNA stability

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

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