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PMID:19965429

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Contents

Citation

Depuydt, M, Leonard, SE, Vertommen, D, Denoncin, K, Morsomme, P, Wahni, K, Messens, J, Carroll, KS and Collet, JF (2009) A periplasmic reducing system protects single cysteine residues from oxidation. Science 326:1109-11

Abstract

The thiol group of the amino acid cysteine can be modified to regulate protein activity. The Escherichia coli periplasm is an oxidizing environment in which most cysteine residues are involved in disulfide bonds. However, many periplasmic proteins contain single cysteine residues, which are vulnerable to oxidation to sulfenic acids and then irreversibly modified to sulfinic and sulfonic acids. We discovered that DsbG and DsbC, two thioredoxin-related proteins, control the global sulfenic acid content of the periplasm and protect single cysteine residues from oxidation. DsbG interacts with the YbiS protein and, along with DsbC, regulates oxidation of its catalytic cysteine residue. Thus, a potentially widespread mechanism controls sulfenic acid modification in the cellular environment.

Links

PubMed Online version:10.1126/science.1179557

Keywords

Amino Acid Sequence; Catalytic Domain; Cysteine/chemistry; Cysteine/metabolism; Disulfides/chemistry; Disulfides/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Models, Biological; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases/chemistry; Oxidoreductases/genetics; Oxidoreductases/metabolism; Periplasm/metabolism; Periplasmic Proteins/chemistry; Periplasmic Proteins/genetics; Periplasmic Proteins/metabolism; Protein Binding; Protein Disulfide-Isomerases/chemistry; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Proteomics; Substrate Specificity; Sulfenic Acids/metabolism

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

dsbG:Gene_Product(s)

GO:0015035

protein disulfide oxidoreductase activity

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

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References

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