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PMID:19966467

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Contents

Citation

Ito, T, Uozumi, N, Nakamura, T, Takayama, S, Matsuda, N, Aiba, H, Hemmi, H and Yoshimura, T (2009) The implication of YggT of Escherichia coli in osmotic regulation. Biosci. Biotechnol. Biochem. 73:2698-704

Abstract

An Escherichia coli mutant lacking three major K(+) uptake systems, Trk, Kup, and Kdp, did not grow under low K(+)and high Na(+) concentrations. The introduction of fkuA and of fkuB of a marine bacterium, Vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of K(+) uptake (Nakamura, Katoh, Shimizu, Matsuba, and Unemoto, Biochim. Biophys. Acta, 1277, 201-208 (1996)). We investigated the function of unknown genes of E. coli, yggS and yggT, homologs of fkuA and fkuB respectively. E. coli TK2420 cells, which lack the three K(+) uptake systems, did not grow under high Na(+) or mannitol concentrations. The growth defect was compensated by the introduction of the yggT gene alone: yggS was not required. Here we found that YggT endowed E. coli cells with a tolerance for osmotic shock, and discuss a possible mechanism.

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Keywords

Amino Acids/metabolism; Cell Proliferation; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Ion Transport; Mutation; Osmotic Pressure; Potassium/metabolism

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

yggT:Gene_Product(s)

GO:0071474

cellular hyperosmotic response

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

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