PortEco logo

PMID:20038592

From EcoliWiki
Jump to: navigation, search

Contents

Citation

Gutgsell, NS and Jain, C (2010) Coordinated regulation of 23S rRNA maturation in Escherichia coli. J. Bacteriol. 192:1405-9

Abstract

In Escherichia coli, rRNAs are transcribed as precursors and require processing at the 3' and 5' ends to generate mature RNA molecules. The largest of these RNAs, 23S rRNA, is matured at the 3' end by a set of exonucleases and at the 5' end by an unknown RNase. Whether the 3' and 5' maturation steps occur independently or are coupled has previously been unclear. By assessing the levels of precursors accumulating at the 3' and 5' ends, we provide evidence that these processes may be linked. Thus, each of several conditions that led to precursor accumulation at one end also did so at the other end. We also observed that each end undergoes maturation at similar rates, suggesting that the two processes could be coupled. Finally, we provide evidence that processing at the 3' end facilitates 5'-end maturation. A model to explain the basis for the observed directionality of the reactions is proposed. This information will aid in the search for the enzyme responsible for final maturation of the 5' end of 23S rRNA.

Links

PubMed PMC2820849 Online version:10.1128/JB.01314-09

Keywords

Base Sequence; Escherichia coli/metabolism; Escherichia coli/physiology; Molecular Sequence Data; Nucleic Acid Conformation; RNA Processing, Post-Transcriptional; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/metabolism

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also

References

See Help:References for how to manage references in EcoliWiki.