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PMID:20054882

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Contents

Citation

Trotter, V, Vinella, D, Loiseau, L, Ollagnier de Choudens, S, Fontecave, M and Barras, F (2009) The CsdA cysteine desulphurase promotes Fe/S biogenesis by recruiting Suf components and participates to a new sulphur transfer pathway by recruiting CsdL (ex-YgdL), a ubiquitin-modifying-like protein. Mol. Microbiol. 74:1527-42

Abstract

Cysteine desulphurases are primary sources of sulphur that can eventually be used for Fe/S biogenesis or thiolation of various cofactors and tRNA. Escherichia coli contains three such enzymes, IscS, SufS and CsdA. The importance of IscS and SufS in Fe/S biogenesis is well established. The physiological role of CsdA in contrast remains uncertain. We provide here additional evidences for a functional redundancy between the three cysteine desulphurases in vivo. In particular, we show that a deficiency in isoprenoid biosynthesis is the unique cause of the lethality of the iscS sufS mutant. Moreover, we show that CsdA is engaged in two separate sulphur transfer pathways. In one pathway, CsdA interacts functionally with SufE-SufBCD proteins to assist Fe/S biogenesis. In another pathway, CsdA interacts with CsdE and a newly discovered protein, which we called CsdL, resembling E1-like proteins found in ubiquitin-like modification systems. We propose this new pathway to allow synthesis of an as yet to be discovered thiolated compound.

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PubMed

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Artificial Gene Fusion; Biosynthetic Pathways; Carrier Proteins/genetics; Carrier Proteins/metabolism; DEAD-box RNA Helicases/metabolism; DNA Transposable Elements; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Genes, Reporter; Iron/metabolism; Lyases/genetics; Lyases/metabolism; Mutagenesis, Insertional; Protein Binding; Protein Interaction Mapping; Sulfur/metabolism; Ubiquitin-Activating Enzymes/metabolism; beta-Galactosidase/genetics; beta-Galactosidase/metabolism

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

csdE:Gene_Product(s)

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

UniProtKB:Q46927

F

Seeded from EcoCyc (v14.0)

complete

ygdL:Gene_Product(s)

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

UniProtKB:P0AGF2

F

Seeded from EcoCyc (v14.0)

complete

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