PortEco logo


From EcoliWiki
Jump to: navigation, search



Köhler, SD, Weber, A, Howard, SP, Welte, W and Drescher, M (2010) The proline-rich domain of TonB possesses an extended polyproline II-like conformation of sufficient length to span the periplasm of Gram-negative bacteria. Protein Sci. 19:625-30


TonB from Escherichia coli and its homologues are critical for the uptake of siderophores through the outer membrane of Gram-negative bacteria using chemiosmotic energy. When different models for the mechanism of TonB mediated energy transfer from the inner to the outer membrane are discussed, one of the key questions is whether TonB spans the periplasm. In this article, we use long range distance measurements by spin-label pulsed EPR (Double Electron-Electron Resonance, DEER) and CD spectroscopy to show that the proline-rich segment of TonB exists in a PPII-like conformation. The result implies that the proline-rich segment of TonB possesses a length of more than 15 nm, sufficient to span the periplasm of Gram-negative bacteria.


PubMed PMC2867004 Online version:10.1002/pro.345


Bacterial Outer Membrane Proteins/chemistry; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Circular Dichroism; Electron Spin Resonance Spectroscopy; Gram-Negative Bacteria/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Peptides/chemistry; Periplasm/metabolism; Proline/chemistry


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.


Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also


See Help:References for how to manage references in EcoliWiki.