Picas, L, Carretero-Genevrier, A, Montero, MT, Vázquez-Ibar, JL, Seantier, B, Milhiet, PE and Hernández-Borrell, J (2010) Preferential insertion of lactose permease in phospholipid domains: AFM observations. Biochim. Biophys. Acta 1798:1014-9
We report the insertion of a transmembrane protein, lactose permease (LacY) from Escherichia coli (E. coli), in supported lipid bilayers (SLBs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), in biomimetic molar proportions. We provide evidence of the preferential insertion of LacY in the fluid domains. Analysis of the self-assembled protein arrangements showed that LacY: (i) is inserted as a monomer within fluid domains of SLBs of POPE:POPG (3:1, mol/mol), (ii) has a diameter of approx. 7.8nm; and (iii) keeps an area of phospholipids surrounding the protein that is compatible with shells of phospholipids.
Escherichia coli/enzymology; Lipid Bilayers/chemistry; Membrane Transport Proteins/chemistry; Microscopy, Atomic Force; Phosphatidylethanolamines/chemistry; Phosphatidylglycerols/chemistry; Phospholipids/chemistry
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
|Gene product||Qualifier||GO ID||GO term name||Evidence Code||with/from||Aspect||Notes||Status|
Add links to pages that link here (e.g. gene, product, method pages)
See Help:References for how to manage references in EcoliWiki.