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PMID:20129058

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Citation

Makowska-Grzyska, M and Kaguni, JM (2010) Primase directs the release of DnaC from DnaB. Mol. Cell 37:90-101

Abstract

An AAA+ ATPase, DnaC, delivers DnaB helicase at the E. coli chromosomal origin by a poorly understood process. This report shows that mutant proteins bearing alanine substitutions for two conserved arginines in a motif named box VII are defective in DNA replication, but this deficiency does not arise from impaired interactions with ATP, DnaB, or single-stranded DNA. Despite their ability to deliver DnaB to the chromosomal origin to form the prepriming complex, this intermediate is inactive. Quantitative analysis of the prepriming complex suggests that the DnaB-DnaC complex contains three DnaC monomers per DnaB hexamer and that the interaction of primase with DnaB and primer formation triggers the release of DnaC, but not the mutants, from DnaB. The interaction of primase with DnaB and the release of DnaC mark discrete events in the transition from initiation to the elongation stage of DNA replication.

Links

PubMed PMC2819048 Online version:10.1016/j.molcel.2009.12.031

Keywords

Adenosine Triphosphate/metabolism; Amino Acid Motifs; Arginine/chemistry; Arginine/physiology; DNA Primase/physiology; DNA Replication/physiology; DNA, Single-Stranded/metabolism; DnaB Helicases/chemistry; DnaB Helicases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Molecular Sequence Data; Protein Interaction Mapping; Replication Origin

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