Yosef, I, Bochkareva, ES, Adler, J and Bibi, E (2010) Membrane protein biogenesis in Ffh- or FtsY-depleted Escherichia coli. PLoS ONE 5:e9130
The Escherichia coli version of the mammalian signal recognition particle (SRP) system is required for biogenesis of membrane proteins and contains two essential proteins: the SRP subunit Ffh and the SRP-receptor FtsY. Scattered in vivo studies have raised the possibility that expression of membrane proteins is inhibited in cells depleted of FtsY, whereas Ffh-depletion only affects their assembly. These differential results are surprising in light of the proposed model that FtsY and Ffh play a role in the same pathway of ribosome targeting to the membrane. Therefore, we decided to evaluate these unexpected results systematically.
Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Western; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Protein Binding; Protein Biosynthesis; Protein Transport; Receptors, Cytoplasmic and Nuclear/genetics; Receptors, Cytoplasmic and Nuclear/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes/metabolism; Signal Recognition Particle/genetics; Signal Recognition Particle/metabolism; Signal Transduction
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