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PMID:21321206

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Contents

Citation

Cho, H, McManus, HR, Dove, SL and Bernhardt, TG (2011) Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist. Proc. Natl. Acad. Sci. U.S.A. 108:3773-8

Abstract

The tubulin-like FtsZ protein initiates assembly of the bacterial cytokinetic machinery by polymerizing into a ring structure, the Z ring, at the prospective site of division. To block Z-ring formation over the nucleoid and help coordinate cell division with chromosome segregation, Escherichia coli employs the nucleoid-associated division inhibitor, SlmA. Here, we investigate the mechanism by which SlmA regulates FtsZ assembly. We show that SlmA disassembles FtsZ polymers in vitro. In addition, using chromatin immunoprecipitation (ChIP), we identified 24 SlmA-binding sequences (SBSs) on the chromosome. Remarkably, SlmA binding to SBSs dramatically enhanced its ability to interfere with FtsZ polymerization, and ChIP studies indicate that SlmA regulates FtsZ assembly at these sites in vivo. Because of the dynamic and highly organized nature of the chromosome, coupling SlmA activation to specific DNA binding provides a mechanism for the precise spatiotemporal control of its anti-FtsZ activity within the cell.

Links

PubMed PMC3048121 Online version:10.1073/pnas.1018674108

Keywords

Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/metabolism; Bacterial Proteins/ultrastructure; Base Sequence; Binding Sites; Carrier Proteins/metabolism; Chromosomes, Bacterial/metabolism; Cytoskeletal Proteins/antagonists & inhibitors; Cytoskeletal Proteins/metabolism; Cytoskeletal Proteins/ultrastructure; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Models, Biological; Molecular Sequence Data; Polymerization; Protein Binding

Significance

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Useful Materials and Methods

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

ftsZ:Gene_Product(s)

GO:0051258

protein polymerization

IDA: Inferred from Direct Assay

P

Figure 3

complete

slmA:Gene_Product(s)

GO:0010974

negative regulation of barrier septum formation

IDA: Inferred from Direct Assay

P

Figure 3 and S3- SlmA destabilizes FtsZ polymers

complete

slmA:Gene_Product(s)

GO:0043547

positive regulation of GTPase activity

IDA: Inferred from Direct Assay

P

Figure 4A- SlmA bound to consensus DNA increases FtsZ's GTPase activity

complete

slmA:Gene_Product(s)

GO:0003677

DNA binding

IDA: Inferred from Direct Assay

F

Figure S7- EMSA

complete

slmA:Gene_Product(s)

GO:0032272

negative regulation of protein polymerization

IDA: Inferred from Direct Assay

P

Figures 1, 3 and S3- interferes with FtsZ polymerization

complete

Data

Strain/Organism Assay Analyte Variable Conditions Notes Links

MG1655

ChIP-chip

SlmA

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See also

References

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