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PMID:2836392

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Contents

Citation

Pargellis, CA, Nunes-Düby, SE, de Vargas, LM and Landy, A (1988) Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine. J. Biol. Chem. 263:7678-85

Abstract

High levels of covalent integrase-DNA complexes accumulate when suicide substrates containing a medial nick within the overlap region are nicked by lambda integrase protein. The tyrosine residue at position 342 is shown to form a covalent bond with DNA at the sites of strand exchange. A mutant integrase in which this tyrosine is changed to phenylalanine is devoid of both topoisomerase and recombinase activity but still binds to both core- and arm-type DNA binding sites with an affinity comparable to wild-type integrase. Tyrosine-342 is located within a 40-amino acid region that is conserved among 15 known recombinases comprising the "integrase family." The present results show that this small region of homology participates in catalysis of strand transfer.

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Keywords

Amino Acid Sequence; Binding Sites; DNA/metabolism; DNA Nucleotidyltransferases/metabolism; DNA Topoisomerases, Type I/metabolism; Integrases; Molecular Sequence Data; Tyrosine/metabolism

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