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Transduction
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Transduction is the transfer of DNA from one bacterium to another by a phage.
Generalized transducing phages accidently package host DNA into a small fraction of the phage capsids produced during lytic infection. Phage P1 is a commonly used generalized transducing phage for E. coli. Any region of the bacterial chromosome can be transferred by a generalized transducing phage.
Specialized transducing phages are phage that have a segment of bacterial DNA integrated into their genome. An example of a specialized transducing phage is lambda dgal, which carries the gal operon from E. coli.
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Generalized transduction in E. coli
Transduction with P1
Protocols for P1 transduction have been reviewed by Thomason, Costantino, and Court[1].
Transduction with T4
Specialized transduction in E. coli
References
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- ↑ Thomason LC et al. (2007) E. coli genome manipulation by P1 transduction. Curr Protoc Mol Biol Chapter 1: Unit 1.17 PubMed EcoliWiki page
