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aas:On One Page

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Gene Name	aas (synonyms: ECK2832, b2836, JW2804)^[1]
Product(s)	Aas product^[1]: fused 2-acylglycerophospho-ethanolamine acyl transferase product, acyl-acyl carrier protein synthetase product, B2836 product
Description	AAS-MONOMER^[2]^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ygeD^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	aas
Mnemonic
Synonyms	ECK2832, b2836, JW2804^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	64.05 minutes	MG1655: 2974036..2971877	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 2974670..2972511	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

64.05 minutes

MG1655: 2974036..2971877
gbrowse_img.php?coord=2970877..2975036&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 2974670..2972511
gbrowse_img.php?coord=2971511..2975670&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
Δaas (Keio:JW2804)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC10191^[5]
Δaas-761::kan			PMID:16738554^[4]	CGSC:99872
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW2804	Plasmid clone	Shigen	PMID:16769691^[6] Status:Clone OK Primer 1:GCCCTTTTTAGCTTTTTTCGAAA Primer 2:CCtTCATCGTGTTGTTCCGCTTC
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: 46% ^[7]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: % ^[7] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11679
EchoBASE (EcoliWiki Page)	EchoBASE:EB1630
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2836
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C3431
EcoGene (EcoliWiki Page)	EcoGene:EG11679
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:AAS
CGSC (EcoliWiki Page)	CGSC:29780
ASAP (EcoliWikiPage)	ASAP:ABE-0009302
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	Aas
Synonyms	fused 2-acylglycerophospho-ethanolamine acyl transferase^[1], acyl-acyl carrier protein synthetase^[1], B2836^[2]^[1], Aas^[2]^[1]
Product description	AAS^[2]^[3]
EC number (for enzymes)	2.3.1.40^[1] 6.2.1.20^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016874	ligase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0436	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0003824	catalytic activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR000873 SP_KW:KW-0511	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016020	membrane	GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005524	ATP binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008152	metabolic process	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000873 InterPro:IPR002123	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008415	acyltransferase activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002123 SP_KW:KW-0012	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008779	acyl-(acyl-carrier-protein)-phospholipid O-acyltransferase activity	GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	EC:2.3.1.40 HAMAP:MF_01162	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008922	long-chain-fatty-acid-(acyl-carrier-protein) ligase activity	GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	EC:6.2.1.20 HAMAP:MF_01162	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006464	protein modification process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006654	phosphatidic acid biosynthetic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MLFSFFRNLC RVLYRVRVTG DTQALKGERV LITPNHVSFI DGILLGLFLP VRPVFAVYTS ISQQWYMRWL KSFIDFVPLD PTQPMAIKHL VRLVEQGRPV VIFPEGRITT TGSLMKIYDG AGFVAAKSGA TVIPVRIEGA ELTHFSRLKG LVKRRLFPQI TLHILPPTQV AMPDAPRARD RRKIAGEMLH QIMMEARMAV RPRETLYESL LSAMYRFGAG KKCVEDVNFT PDSYRKLLTK TLFVGRILEK YSVEGERIGL MLPNAGISAA VIFGAIARRR MPAMMNYTAG VKGLTSAITA AEIKTIFTSR QFLDKGKLWH LPEQLTQVRW VYLEDLKADV TTADKVWIFA HLLMPRLAQV KQQPEEEALI LFTSGSEGHP KGVVHSHKSI LANVEQIKTI ADFTTNDRFM SALPLFHSFG LTVGLFTPLL TGAEVFLYPS PLHYRIVPEL VYDRSCTVLF GTSTFLGHYA RFANPYDFYR LRYVVAGAEK LQESTKQLWQ DKFGLRILEG YGVTECAPVV SINVPMAAKP GTVGRILPGM DARLLSVPGI EEGGRLQLKG PNIMNGYLRV EKPGVLEVPT AENVRGEMER GWYDTGDIVR FDEQGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSPDK VHATAIKSDA SKGEALVLFT TDNELTRDKL QQYAREHGVP ELAVPRDIRY LKQMPLLGSG KPDFVTLKSW VDEAEQHDE
Mol. Wt	80.7 kDa (calc) ^[2]
pI	9.37^[2]
Extinction coefficient	76780 - 77280 (calc based on 22 Y, 8 W, and 4 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative homolog: 1PG3\|A (Salmonella enterica) 244-577 of 719 residues (E-value: 1.1e-06) (Percent Identity: 24.20) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
1PG3|A (Salmonella enterica)
244-577 of 719 residues (E-value: 1.1e-06) (Percent Identity: 24.20)
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Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:AAS-MONOMER
UniProt (EcoliWiki Page)	UniProt:P31119
PFAM (EcoliWiki Page)	PFAM:PF01553
RefSeq (EcoliWiki Page)	RefSeq:NP_417313
ModBase (EcoliWiki Page)	ModBase:P31119
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
ygeD
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Figure courtesy of RegulonDB

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2974016..2974056&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b2836 (EcoliWiki Page)	Summary of data for aas from multiple microarray studies
microarray	GEO Profiles:b2836 (EcoliWiki Page)	NCBI GEO profiles for aas
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aas Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11679
EchoBASE (EcoliWiki Page)	EchoBASE:EB1630
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b2836
EcoGene (EcoliWiki Page)	EcoGene:EG11679
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Shigella flexneri	AAS	From SHIGELLACYC
E. coli O157	AAS	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF01553
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.00 ^8.01 ^8.02 ^8.03 ^8.04 ^8.05 ^8.06 ^8.07 ^8.08 ^8.09 ^8.10 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/aas:On_One_Page"

aas:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aas Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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