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aat:On One Page

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Gene Name	aat (synonyms: ECK0876, b0885, JW0868, ycaA, G8002)^[1]^[2]
Product(s)	Aat product^[1]: leucyl/phenylalanyl-tRNA-protein transferase product, B0885 product, YcaA product
Description	leucyl, phenylalanyl-tRNA-protein transferase^[2]^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aat^[2]
Regulation/Activity
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Gene

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Nomenclature

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Standard name	aat
Mnemonic
Synonyms	ECK0876, b0885, JW0868, ycaA, G8002^[1]^[2]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	19.96 minutes	MG1655: 926655..925951	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 927854..927150	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

19.96 minutes

MG1655: 926655..925951
gbrowse_img.php?coord=924951..927655&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 927854..927150
gbrowse_img.php?coord=926150..928854&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
Δaat (Keio:JW0868)	deletion	deletion			PMID:16738554^[4]	Shigen CGSC8899^[5]
aat-1			Other	MS845 has only 50% the tryptophanase activity of parent strain.	PMID:137233^[6]	CGSC:8542
Δaat-784::kan					PMID:16738554^[4]	CGSC:99874
aat-1			Cell Shape	Cells appear as clubs,spheres, or ellipsoids. Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic acid to the medium.	PMID:137233^[6]	CGSC:8542
aat-1			Growth Phenotype	When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively).	PMID:137233^[6]	CGSC:8542
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW0868	Plasmid clone	Shigen	PMID:16769691^[7] Status:Clone OK Primer 1:GCCCGCCTGGTTCAGCTTTCTCG Primer 2:CCTTCTTGTGGTGAAAACAAGCA
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: % ^[8] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 47% ^[8] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11112
EchoBASE (EcoliWiki Page)	EchoBASE:EB1103
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0885
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C1022
EcoGene (EcoliWiki Page)	EcoGene:EG11112
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:AAT
ASAP (EcoliWikiPage)	ASAP:ABE-0003009
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	Aat
Synonyms	leucyl/phenylalanyl-tRNA-protein transferase^[1], B0885^[2]^[1], YcaA^[2]^[1], Aat^[2]^[1]
Product description	leucyl, phenylalanyl-tRNA-protein transferase^[2]^[3]
EC number (for enzymes)	2.3.2.6^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0008415	acyltransferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0012	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0008914	leucyltransferase activity	GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	EC:2.3.2.6 HAMAP:MF_00688	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0016755	transferase activity, transferring amino-acyl groups	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004616	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0030163	protein catabolic process	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR004616 HAMAP:MF_00688	P	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0006508	proteolysis				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0009050	glycopeptide catabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF SPGDPILWWS PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE GCASDREEGT WITRGVVEAY HRLHELGHAH SIEVWREDEL VGGMYGVAQG TLFCGESMFS RMENASKTAL LVFCEEFIGH GGKLIDCQVL NDHTASLGAC EIPRRDYLNY LNQMRLGRLP NNFWVPRCLF SPQE
Mol. Wt	26.62 kDa (calc) ^[2]
pI
Extinction coefficient	48930 - 49680 (calc based on 7 Y, 7 W, and 6 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative Escherichia coli structure: 2Z3L\|A 1-233 of 234 residues (E-value: 2.0e-130) (Percent Identity: 100.00) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative Escherichia coli structure:
2Z3L|A
1-233 of 234 residues (E-value: 2.0e-130) (Percent Identity: 100.00)
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Models

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11112-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0A8P1
PFAM (EcoliWiki Page)	PFAM:PF03588
RefSeq (EcoliWiki Page)	RefSeq:NP_415405
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
aat
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=926635..926675&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b0885 (EcoliWiki Page)	Summary of data for aat from multiple microarray studies
microarray	GEO Profiles:b0885 (EcoliWiki Page)	NCBI GEO profiles for aat
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aat Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11112
EchoBASE (EcoliWiki Page)	EchoBASE:EB1103
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b0885
EcoGene (EcoliWiki Page)	EcoGene:EG11112
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000012980 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AAT	From SHIGELLACYC
E. coli O157	AAT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF03588
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 Deutch CE et al. (1977) Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J Bacteriol 129: 544-6 PubMed EcoliWiki page
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^8.0 ^8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^9.0 ^9.1 ^9.2 ^9.3 ^9.4 ^9.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/aat:On_One_Page"

aat:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

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Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aat Expression

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Links

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Evolution

Homologs in Other Organisms

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